Method and apparatus for high speed carbohydrate analysis
View Patent ↗A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, high speed, high throughput, carbohydrate analysis. In one aspect of the present invention, a high-performance capillary electrophoresis analyzer system has been optimized for carbohydrate analysis application. The system uses a multiplexed/time-staggered fluorescence type detection mechanism, with an integrated fiber optic array-based technology and a novel disposable gel-cartridge. Twelve carbohydrate samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available labeling agent such as APTS (representing 8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt) as an indicator, the capillary electrophoresis-based carbohydrate system/analyzer provides high resolving power in 2-15 minutes of separations. The system can hold a total of 96 samples, which can be automatically analyzed within 30-60 minutes. This affordable fiber optic based fluorescence detection system can be used in laboratories for high speed carbohydrate sequencing & screening applications.
1. A cartridge for bio-analysis, comprising:
a body;
a plurality of capillaries supported by the body;
a reservoir common to the capillaries; and
a linear gel matrix formulated for electrophoretic analysis of carbohydrate contained in the reservoir, wherein the gel matrix comprises a linear polymer solution as a gel sieving matrix, wherein the linear polymer solution comprises:
—
mwt g/mol
Concentration
PEO
7000000
0.000607
mM
PEO
900000
0.002777778
mM
MOPS
—
12.5
mM
Tris
—
7.85
mM
NP40
—
0.05%
NaN3
—
1
mM
EtBr
—
10
mM.
2. A bio-analysis system, comprising:
a base;
a cartridge as in claim 1 ;
a cartridge interface support on the base, interfacing with the cartridge system; and
a controller operatively coupled to the cartridge interface to control operations of the cartridge interface.
3. The cartridge of claim 1 , wherein the gel matrix comprises a linear polymer solution as a gel sieving matrix, wherein the linear polymer solution comprises between about 0.01% and about 1.5% polyethylene oxide (“PEO”); between about 0.0% and less than about 2.0% of a second polyethylene oxide (“PEO”); between about 0.0% and about 2.0% of a surfactant; between about 0.0% and about 99% of a polyol; and between about 0.0M and about 1.0M of a pH buffer, where the composition has a pH of between about 2.0 and about 10.0.
4. A method of carbohydrate detection/analysis, comprising:
providing a cartridge, which comprises:
a body;
a plurality of capillaries supported by the body;
a reservoir common to the capillaries; and
a linear gel matrix formulated for electrophoretic analysis of carbohydrate contained in the reservoir;
introducing a carbohydrate sample into one end of each capillary;
undertaking electropherisis separation along each capillary, to separate the carbohydrate sample into components to be analyzed
wherein the gel matrix comprises a linear polymer solution as a gel sieving matrix, wherein the linear polymer solution comprises:
—
mwt g/mol
Concentration
PEO
7000000
0.000607
mM
PEO
900000
0.002777778
mM
MOPS
—
12.5
mM
Tris
—
7.85
mM
NP40
—
0.05%
NaN3
—
1
mM
EtBr
—
10
mM.
5. The method of claim 4 , wherein the gel matrix comprises a linear polymer solution as a gel sieving matrix, wherein the linear polymer solution comprises between about 0.01% and about 1.5% polyethylene oxide (“PEO”); between about 0.0% and less than about 2.0% of a second polyethylene oxide (“PEO”); between about 0.0% and about 2.0% of a surfactant; between about 0.0% and about 99% of a polyol; and between about 0.0M and about 1.0M of a pH buffer, where the composition has a pH of between about 2.0 and about 10.0.
6. A cartridge for bio-analysis, comprising:
a body;
a plurality of capillaries supported by the body;
a reservoir common to the capillaries; and
a gel matrix formulated for electrophoretic analysis of carbohydrate contained in the reservoir, wherein the gel matrix comprises a polymer solution as a gel sieving matrix, wherein the polymer solution comprises first and second long polyethylene oxide (PEO) polymers having substantially different molecular weights, each in a very small concentration, wherein the first and second long PEO polymers are about 7.8 times different in molecular weights.
7. The cartridge of claim 6 , wherein the first and second long PEO polymers have different concentrations.
8. The cartridge of claim 7 , wherein the concentrations of the first and second long PEO polymers are substantially different.
9. The cartridge of claim 6 , wherein the concentration of the first long PEO polymer is about 0.000607mM.
10. The cartridge of claim 9 , wherein the concentration of the second long PEO polymer is about 0.002777778mM.
11. The cartridge of claim 6 , wherein the concentration of the second long PEO polymer is about 0.002777778mM.
12. The cartridge of claim 6 , wherein the molecular weight of the first long PEO polymer is about 7000000 mwt g/mol.
13. The cartridge of claim 12 , wherein the molecular weight of the second long PEO polymer is about 900000 mwt g/mol.
14. The cartridge of claim 6 , wherein the molecular weight of the second long PEO polymer is about 900000 mwt g/mol.
15. The cartridge of claim 6 , wherein the molecular weight of the first long PEO polymer is larger than the molecular weight of the second long PEO polymer, and the concentration of the first long PEO polymer is smaller than the concentration of the second long PEO polymer.
16. The cartridge of claim 15 , wherein the concentrations of the first and second long PEO polymers are substantially different.
17. The cartridge of claim 16 , wherein the first long PEO polymer has a molecular weight of substantially 7000000 mwt g/mol and a concentration of substantially 0.000607mM, and the second long PEO polymer has a molecular weight of substantially 900000 mwt g/mol and a concentration of substantially 0.002777778mM.
18. The cartridge of claim 6 , wherein the first and second long PEO polymers are linear polymers.
19. The cartridge of claim 6 , wherein the gel matrix comprises a linear polymer solution as a gel sieving matrix, wherein the linear polymer solution comprises between about 0.01% and about 1.5% of the first long PEO polymer; between about 0.0% and less than about 2.0% of the second long PEO polymer; between about 0.0% and about 2.0% of a surfactant;
between about 0.0% and about 99% of a polyol; and between about 0.0M and about 1.0M of a pH buffer, where the composition has a pH of between about 2.0 and about 10.0.
20. A method of carbohydrate detection/analysis, comprising:
providing a cartridge, which comprises:
a body;
a plurality of capillaries supported by the body;
a reservoir common to the capillaries; and
a gel matrix formulated for electrophoretic analysis of carbohydrate contained in the reservoir;
introducing a carbohydrate sample into one end of each capillary;
undertaking electropherisis separation along each capillary, to separate the carbohydrate sample into components to be analyzed, wherein the gel matrix comprises a polymer solution as a gel sieving matrix, wherein the polymer solution comprises first and second long PEO polymers having substantially different molecular weights, each in a very small concentration, wherein the first and second long PEO polymers are about 7.8 times different in molecular weights.