Granted Patent B2

US 7,723,487 · App. 11/983,939 · Granted May 25, 2010

Abstract

Claims (5)

1. A method for producing an antibody that binds cancer specific Proliferating Cell Nuclear Antigen (csPCNA), comprising:

(A) obtaining a tissue or body fluid sample comprising csPCNA;

(B) contacting said sample with a peptide comprising the amino acid sequence LeuLysGlnLeuAspAlaGlnGlnThrGlnLeuArgIleAspSerPhePheArgLeuAlaGlnGlnGluLysGluAspAlaLysArg (SEQ ID NO:1), wherein said peptide is immobilized on a solid support and binds to said csPCNA to form a peptide-csPCNA complex;

(C) isolating csPCNA from said peptide-csPCNA complex so as to purify said csPCNA; and

(D) producing an antibody using said csPCNA isolated from said peptide-csPCNA complex.

Assignments (6)