Nucleic Acids Encoding a Functional Mammalin Purinoreceptor, P2X3, Methods of Production and Use Thereof
The subject invention relates to the rhesus monkey P 2 X 3 receptor, nucleic acids encoding this receptor, methods of modulating the activity of a target purinergic receptor using the P 2 X 3 receptor and to uses of these methods. In particular, such methods may be used, for example, to accelerate the rate of resensitization of a desensitized receptor.
1 .- 15 . (canceled)
16 . An isolated polynucleotide encoding a rhesus P 2 X 3 receptor protein comprising the amino acid sequence of SEQ ID NO.:2.
17 . An isolated polynucleotide encoding a receptor protein of claim 16 , or a functionally active fragment thereof, wherein said polynucleotide comprises a sequence selected from the group consisting of:
(a) a sequence of nucleotides that encodes the amino acid sequence set forth in SEQ ID NO.:2, or a functionally active fragment thereof;
(b) a sequence of nucleotides as set forth in SEQ ID NO.:1; and
(c) a sequence of nucleotides that encode a mammalian purinergic receptor subtype and that hybridizes to the sequence of nucleotides of (a) or (b) under moderately stringent conditions, wherein said sequence of nucleotides encodes a biologically active mammalian purinergic receptor subtype designated as P 2 X 3 herein.
18 . An expression vector comprising a polynucleotide of claim 17 .
19 . A host cell transformed or transfected with the expression vector of claim 18 .
20 . A host cell of claim 19 , wherein said cell is selected from the group consisting of a bacterial cell, a mammalian cell, a yeast cell and an amphibian cell.
21 . A host cell according to claim 20 , wherein the cell is an amphibian cell.
22 . A host cell according to claim 20 , wherein the cell is a mammalian cell.
23 . A method for producing a rhesus P 2 X 3 receptor polypeptide, the method comprising the steps of:
(a) transfecting or transforming a suitable host cell with the expression vector of claim 18 ;
(b) culturing the host cells of step (a) under conditions which allow expression of the recombinant receptor polypeptide from the expression vector; and
(c) harvesting the recombinant receptor polypeptide from the culture, culturing a host.