Circovirus sequences associated with piglet weight loss disease (PWD)
View Patent ↗The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection are further provided. Pharmaceutical, including vaccine, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.
1. A baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide whose nucleotide sequence has at least 90% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
2. The baculovirus of claim 1 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 95% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
3. The baculovirus of claim 1 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 98% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
4. A method for producing an immunogenic polypeptide encoded by a nucleotide sequence having at least 90% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25) comprising transforming a host cell with the baculovirus of claim 1 and culturing the transformed host cell under conditions whereby the immunogenic polypeptide is expressed.
5. The method of claim 4 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 95% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
6. The method of claim 4 , wherein the nucleotide sequence that encodes the immunogenic polypeptide has at least 98% identity to the nucleotide sequence encoding ORF′2 (SEQ ID NO:25).
7. A baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide comprising at least 5 contiguous amino acids of ORF′2 (SEQ ID NO:26).
8. The baculovirus of claim 7 , wherein the immunogenic polypeptide comprises at least 10 contiguous amino acids of ORF′2 (SEQ ID NO:26).
9. The baculovirus of claim 7 , wherein the immunogenic polypeptide comprises at least 15 contiguous amino acids of ORF′2 (SEQ ID NO:26).
10. The baculovirus of claim 7 , wherein the immunogenic polypeptide comprises at least 25 contiguous amino acids of ORF′2 (SEQ ID NO:26).
11. A host cell transformed with the baculovirus of claim 7 .
12. A method for producing an immunogenic polypeptide comprising at least 5 contiguous amino acids of ORF′2 (SEQ ID NO:26) comprising transforming a host cell with the baculovirus of claim 7 and culturing the transformed host cell under conditions whereby the immunogenic polypeptide is expressed.
13. The method of claim 12 , wherein the immunogenic polypeptide comprises at least 10 contiguous amino acids of ORF′2 (SEQ ID NO:26).
14. The method of claim 12 , wherein the immunogenic polypeptide comprises at least 15, contiguous amino acids of ORF′2 (SEQ ID NO:26).
15. The method of claim 12 , wherein the immunogenic polypeptide comprises at least 25 contiguous amino acids of ORF′2 (SEQ ID NO:26).
16. The method of claim 12 , further comprising isolating the immunogenic polypeptide from a cell lysate of the transformed host cell.
17. The method of claim 16 , further comprising purifying the immunogenic polypeptide.
18. The method of claim 12 , further comprising purifying the immunogenic polypeptide from media in which the transformed host cell was cultured.
19. A method for reducing viral load of porcine circovirus-B (PCV-B) in a pig, comprising inducing an immunological or immunogenic response against PCV-B in the pig comprising administering to the pig the immunogenic polypeptide produced by the method of any one of claims 12 or 16 - 18 .
20. A baculovirus containing and expressing an exogenous nucleotide sequence, wherein the nucleotide sequence encodes an immunogenic polypeptide having at least 90% identity to ORF′2 (SEQ ID NO:26).
21. The baculovirus of claim 20 , wherein the immunogenic polypeptide has at least 95% identity to ORF′2 (SEQ ID NO:26).
22. The baculovirus of claim 20 , wherein the immunogenic polypeptide has at least 98% identity to ORF′2 (SEQ ID NO:26).
23. A host cell transformed with the baculovirus of claim 1 or claim 20 .
24. A method for producing an immunogenic polypeptide having at least 90% identity to ORF′2 (SEQ ID NO:26) comprising transforming a host cell with the baculovirus of claim 20 and culturing the transformed host cell under conditions whereby the immunogenic polypeptide is expressed.
25. The method of claim 24 , wherein the immunogenic polypeptide has at least 95% identity to ORF′2 (SEQ ID NO:26).
26. The method of claim 24 , wherein the immunogenic polypeptide has at least 98% identity to ORF′2 (SEQ ID NO:26).
27. The method of claim 4 or claim 24 , further comprising isolating the immunogenic polypeptide from a cell lysate of the transformed host cell.
28. The method of claim 27 , further comprising purifying the immunogenic polypeptide.
29. The method of claim 4 or claim 24 , further comprising purifying the immunogenic polypeptide from media in which the transformed host cell was cultured.
30. A method for reducing viral load of porcine circovirus-B (PCV-B) in a pig, comprising inducing an immunological or immunogenic response against PCV-B in the pig comprising administering to the pig the immunogenic polypeptide produced by the method of any one of claims 4 , 27 , 28 , 29 , and 24 .