Purification of proteins
View Patent ↗The present invention relates to a selectively soluble polymer capable of binding to a desired biomolecules in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and/or salt concentration and is rendered insoluble and precipitates out of solution upon a change in the process conditions. The polymer is capable of binding to the desired biomolecule (protein, polypeptide, etc) and remains capable of binding to that biomolecule even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered such as by elution and further processed.
1. A method for purifying a biomolecule from a mixture containing impurities comprising:
a. providing the mixture at a set of pH conditions,
b. adding to said mixture one or more polymers selected from the group consisting of polyvinylpyridine and copolymers of vinylpyridine, soluble in said mixture under the set of pH conditions and capable of reversibly and selectively binding to the biomolecule,
c. mixing the one or more solubilized polymers throughout the mixture;
d. precipitating the one or more polymers by changing the set of pH conditions in the mixture, such that the precipitated polymers bind selectively and reversibly to the biomolecule; and
e. separating the precipitated polymer and bound biomolecule from the mixture and wherein the biomolecule is a monoclonal antibody.
2. The method of claim 1 wherein in step b, the polymer is added to a solution under a set of pH conditions within the solution and the solution containing the solubilized polymer is added to the mixture.
3. The method of claim 1 further comprising step f wherein the biomolecule is recovered from the polymer.
4. The method of claim 3 further comprising the step of incorporating the recovered biomolecule into a pharmaceutical formulation.
5. The method of claim 3 further comprising formulating the recovered biomolecule in a pharmaceutically acceptable carrier.
6. The method of claim 3 further comprising formulating the recovered biomolecule in a pharmaceutically acceptable carrier for a purpose selected from the group consisting of research, diagnostic and therapeutic purposes.
7. The method of claim 1 wherein the one or more polymers is precipitated by a change in pH that is greater or less than 0.1 pH units from the pH level at which the polymer is soluble in the mixture.
8. The method of claim 1 wherein the one or more polymers are solubilized at a pH level of less than about 5.
9. The method of claim 1 wherein monoclonal antibody is a recombinant the monoclonal antibody.
10. The method of claim 1 wherein the one or more polymers are copolymers of vinylpyridine.
11. The method of claim 1 further comprising, prior to step b., adding the one or more polymers to a carrier liquid under conditions to cause the one or more polymers to go into solution and adding the carrier liquid and the one or more polymers in solution to the mixture through a static mixer.
12. The method of claim 1 wherein the one or more polymers are added in excess to the mixture and recovered as the precipitate.
13. The method of claim 1 further comprising the step in which the biomolecule is recovered from the polymer and the recovered biomolecule has at least 1 LRV reduction in impurities over the starting mixture.
14. The method of claim 1 wherein the one or more polymers is poly(4-vinyl pyridine-co-styrene).
15. The method of claim 1 , wherein the pH in step d is above 5.