Microinjection devices and methods of use
The present invention provides for microinjection devices comprising a needle and a viewing instrument wherein the viewing instrument provides viewing of an object to an operator from an angle other than a right angle to the object.
1 . A microinjection device comprising a needle and a viewing instrument wherein the viewing instrument provides magnified viewing of an object from an angle other than a right angle to the object.
2 . The device of claim 1 wherein the needle is hollow.
3 . The device of claim 1 wherein the needle comprises glass.
4 . The device of claim 1 comprising a laser light source.
5 . The device of claim 4 wherein the laser light source illuminates the needle.
6 . The device of claim 4 wherein the laser light travels down the needle.
7 . The device of claim 1 wherein the needle includes a bevel.
8 . The device of claim 1 comprising an injector.
9 . The device of claim 1 comprising an oscillator.
10 . The device of claim 9 wherein the oscillator imparts an oscillation to the needle.
11 . The device of claim 10 wherein the oscillation of the needle has an amplitude of between about 0.001 nm and about 100 μm.
12 . The device of claim 1 wherein the angle is between about 1° and about 89°.
13 . The device of claim 1 wherein the viewing instrument comprises a lens.
14 . The device of claim 1 wherein the viewing instrument includes a borescope.
15 . A microinjection device comprising a needle and a viewing instrument wherein the viewing instrument provides magnified viewing of an object from an angle between about 10° and about 70° to the object.
16 . The device of claim 15 wherein the needle is hollow.
17 . The device of claim 15 wherein the needle comprises glass.
18 . The device of claim 15 comprising a laser light source.
19 . The device of claim 18 wherein the laser light source illuminates the needle.
20 . The device of claim 18 wherein the laser light travels down the needle.
21 . The device of claim 15 wherein the needle includes a bevel.
22 . The device of claim 15 comprising an injector.
23 . The device of claim 15 comprising an oscillator.
24 . The device of claim 15 wherein the viewing instrument comprises a lens.
25 . The device of claim 15 wherein the viewing instrument includes a borescope.
26 . A method comprising:
viewing a germinal disc under magnification at an angle to the germinal disc of less than 90°; and
injecting an artificial chromosome to the germinal disc through a needle.