Disposable bioreactor system
A cylindrical or annular polymeric bioreactor is disclosed which provides enhanced mixing and aeration of the growth medium while simultaneously offering reduced mechanical shear force.
1. An apparatus comprising:
(a) a hollow toroidal one-piece polymeric bioreactor vessel comprising at least one port configured to receive at least one pre-inserted and pre-calibrated sensor to monitor a bioprocess carried out in said hollow toroidal one-piece polymeric bioreactor vessel; and
(b) a drive configured to move the hollow toroidal one-piece polymeric bioreactor vessel such that there is build-up of a resonant frequency traveling wave of fluid orbiting in one direction in an interior of the hollow toroidal one-piece polymeric vessel when a particular amplitude and orbital speed is imparted to said hollow toroidal one-piece polymeric bioreactor vessel,
wherein the resonant frequency depends on the size and shape of the hollow toroidal one-piece polymeric bioreactor vessel, and
wherein at least a portion of an inner surface of said hollow toroidal one-piece polymeric bioreactor vessel is textured with a plurality of upstanding protuberances and said hollow toroidal one-piece polymeric bioreactor vessel includes at least one exit port for the removal of gaseous by-products.
2. The apparatus in accordance with claim 1 , wherein at least some of said upstanding protuberances allow passage of sparging gas into said hollow toroidal one-piece polymeric bioreactor vessel.
3. The apparatus in accordance with claim 2 , having the textured surface on a bottom of the hollow toroidal one-piece polymeric bioreactor vessel.
4. The apparatus in accordance with claim 1 , further comprising pre-molded baffles on at least a portion of the inner surface of said hollow toroidal one-piece polymeric bioreactor vessel.
5. The apparatus in accordance with claim 1 , wherein the apparatus is fabricated from a substantially rigid plastic.
6. The apparatus in accordance with claim 1 , wherein the apparatus is fabricated from a substantially flexible plastic.
7. The apparatus in accordance with claim 1 , further comprising means for illuminating the contents of said hollow toroidal one-piece polymeric bioreactor vessel.
8. The apparatus in accordance with claim 7 wherein said illumination means comprises at least one LED.
9. The apparatus in accordance with claim 1 , further comprising means for simultaneously both orbiting and rocking said hollow toroidal one-piece polymeric bioreactor vessel.