Disposable mixing system
View Patent ↗The present invention uses one or more bags that are capable of being selectively pressurized and deflated in conjunction with a disposable bio bag such as a fermenter, mixing bag, storage bag and the like. The pressure bag(s) may surround a selected outer portion of the bag or may be contained within an inner portion of such a bag. By selectively pressurizing and deflating the pressure bag(s), one is able to achieve fluid motion in the bag thereby ensuring cell suspension, mixing and gas transfer within the bag without damaging shear forces or foam generation.
1. A mixing device consisting essentially of a mixing bag having an inner volume, the inner volume containing a liquid to mixed, one or more pressure bags being in a position within the mixing bag, each of the one or more pressure bags having an inlet, each of the one or more pressure bags having an outlet, the inlet of the one or more pressure bags being connected to a source of pressurized gas and being capable of being selectively opened and closed so as to inflate and deflate the one or more pressure bags as desired.
2. The device of claim 1 wherein the one or more pressure bags are contained within the volume of the bag, are two in number and arranged such that one is adjacent each end of the bag.
3. The device of claim 1 wherein the one or more pressure bags are contained within the volume of the bag at a central location.
4. The device of claim 1 wherein the one or more pressure bags are one in number, are contained within the volume of the bag at a central location and the pressure bag contains a conduit through the pressure bag to allow for flow of fluid from end of the bag volume to the other through the pressure bag.
5. The device of claim 1 wherein the one or more pressure bags is one in number and is located at one end of the mixing bag.
6. The device of claim 1 wherein the inlet and/or outlet of the one or more pressure bags is selectively opened and closed by the one or more valves.
7. The device of claim 1 wherein the one or more pressure bags have an outer surface and the outer surface of the one or more pressure bags is secured to an inner wall of the bag.
8. A system for the mixing of components comprising a bag having a volume for containing the components to be mixed, one or more components, at least one of the one or more components being in liquid form, the bag having an inlet and an outlet, the inlet and outlet being capable of being selectively opened and dosed, one or more pressure bags being positioned within the mixing bag, the one or more pressure bags having an inlet and an outlet, the inlet and outlet of the one or more pressure bags extending through the wall of the bag through openings with a liquid proof seal around the one or more pressure bag inlet and outlet, the inlet of the pressure bag being connected to a source of pressurized fluid, at least the inlet being capable of being selectively opened and closed so as to inflate and deflate the pressure bags as desired and a control unit connected to at least the pressure bag inlet to control the selective opening and closing of the inlet.
9. The system of claim 8 wherein the inlet to the one or more pressure bags has a valve actuated by the control unit.
10. The system of claim 8 wherein the inlet to the one or more pressure bags has a valve actuated by the control unit and the valve is selected from the group consisting of solenoid actuated and pneumatically actuated valves.
11. The system of claim 8 wherein the one or more pressure bags have an outer surface and the outer surface of the one or more pressure bags is secured to an inner wall of the bag.