Methods of protein production in cell lines
View Patent ↗The present invention relates to cells that can be passaged in culture and can be used for, among other things, promoter assays and the production of heterologous proteins.
1. A method comprising culturing under suitable nutrient conditions a hybrid cell comprising an avian tubular gland cell (TGC) fused to an immortal cell, wherein said hybrid cell comprises an exogenous nucleic acid sequence encoding a protein operably linked to an oviduct specific promoter.
2. The method of claim 1 wherein the hybrid cell is sustainable in culture for at least ten or more passages.
3. The method of claim 1 wherein the hybrid cell is sustainable in culture for at least twenty or more passages.
4. The method of claim 1 , wherein the protein is a human protein.
5. The method of claim 1 , wherein the protein is a marker protein.
6. The method of claim 1 wherein the oviduct specific promoter is selected from the group consisting promoters for ovalbumin, ovomucoid, conalbumin, ovomucin and lysozyme proteins.
7. The method of claim 1 , wherein the tubular gland cell and the immortal cell are fused using polyethylene glycol.
8. The method of claim 1 , wherein the hybrid cell is of a hybrid cell line.
9. A method comprising: i) culturing under suitable nutrient conditions a hybrid cell comprising an avian tubular gland cell (TGC) fused to an immortal cell, wherein said hybrid cell comprises an exogenous nucleic acid sequence encoding a therapeutic protein operably linked to an oviduct specific promoter, wherein the hybrid cell secretes the therapeutic protein; and ii) isolating the therapeutic protein.
10. The method of claim 9 wherein the hybrid cell is sustainable in culture for at least ten or more passages.
11. The method of claim 9 wherein the hybrid cell is sustainable in culture for at least twenty or more passages.
12. The method of claim 9 wherein the oviduct specific promoter is a promoter for ovomucin protein.
13. The method of claim 9 wherein the oviduct specific promoter is a promoter for ovalbumin protein.
14. The method of claim 9 wherein the oviduct specific promoter is a promoter for ovomucoid protein.
15. The method of claim 9 wherein the oviduct specific promoter is a promoter for lysozyme protein.
16. The method of claim 9 wherein the oviduct specific promoter is a promoter for conalbumin protein.
17. The method of claim 9 wherein the therapeutic protein is selected from the group consisting of antibodies, cytokines, fusion proteins, growth factors, enzymes, interferons, granulocyte-colony stimulating factors, and erythropoietins.
18. The method of claim 9 , wherein the hybrid cell is of a hybrid cell line.