Use of untranslated region of osmotin gene to enhance transgene expression in plants
View Patent ↗The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5′ and 3′ untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
1. A nucleic acid construct comprising at least one structural gene of interest functionally linked to a heterologous promoter and one or more of an isolated osmotin UTR consisting of a nucleic acid sequence selected from the group consisting of SEQ. ID. No. 1, SEQ. ID. No. 2, and SEQ. ID. No. 3.
2. The nucleic acid construct of claim 1 , wherein the at least one structural gene of interest comprises a gene that confers a non-native phenotype in a plant.
3. The nucleic acid construct of claim 1 , wherein the at least one structural gene of interest comprises a gene that confers insecticide or herbicide resistance in a plant.
4. The construct of claim 1 , wherein the at least one structural gene of interest comprises SEQ. ID. No. 5.
5. A plant or plant cell transformed with the nucleic acid construct of claim 1 .
6. The plant or plant cell of claim 5 further comprising an additional structural gene of interest stacked with the at least one gene of interest.
7. The plant or plant cell of claim 6 , wherein the additional structural gene of interest is functionally linked to a heterologous promoter and one or more of an isolated osmotin UTR consisting of a nucleic acid sequence selected from the group consisting of SEQ. ID. No. 1, SEQ. ID. No. 2, and SEQ. ID. No. 3.
8. A vector comprising the nucleic acid construct of claim 1 .