Wuschel (WUS) gene homologs
View Patent ↗This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.
1. An isolated polynucleotide comprising a nucleotide sequence encoding a WUSCHEL polypeptide containing a homeodomain, wherein the polypeptide has an amino acid sequence of at least 95% sequence identity over its entire length to SEQ ID NO: 6 or SEQ ID NO: 8 based on the CLUSTAL alignment method using default parameters of KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, and wherein the polypeptide is capable of stimulating in vitro growth of plant tissue.
2. The isolated polynucleotide of claim 1 , wherein the WUSCHEL polypeptide comprises the amino acid sequence of SEQ ID NO: 6 or SEQ ID NO: 8.
3. The isolated polynucleotide of claim 1 , wherein the nucleotide sequence comprises SEQ ID NO: 5 or SEQ ID NO: 7.
4. A transgenic plant or transgenic seed therefrom comprising the polynucleotide of claim 1 .
5. The plant of claim 4 , wherein the plant is selected from the group consisting of corn, soybean, wheat, rice, alfalfa, sunflower, canola, and cotton.
6. A method for inducing meristem proliferation in a plant cell comprising:
(a) introducing into a plant cell the polynucleotide of claim 1 operably linked to a regulatory sequence operable in the plant cell; and,
(b) expressing the polynucleotide for a time sufficient to induce meristem proliferation.
7. The method of claim 6 wherein the polynucleotide is integrated into the plant cell genome to produce a transformed plant cell comprising the polynucleotide.
8. The method of claim 7 further comprising growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
9. A method for positive selection of a transformed cell, comprising:
(a) introducing into a plant cell the polynucleotide of claim 1 operably linked to a regulatory sequence operable in the plant cell; and,
(b) expressing the polynucleotide for a time sufficient to induce organogenesis and provide a positive selection means.
10. The method of claim 9 further comprising growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
11. A method to stimulate plant cell growth, wherein the method comprises:
(a) introducing into a plant cell the isolated polynucleotide of claim 1 operably linked to a regulatory sequence operable in the plant cell; and,
(b) expressing the polynucleotide in the plant cell;
wherein plant cells expressing the polynucleotide show stimulated plant cell growth as compared to control plant cells that do not have or express the polynucleotide.
12. The method of claim 11 further comprising growing the plant cell under plant growing conditions to produce a regenerated plant.