Cucumbers comprising DNA from PI 250147 conferring closterovirus resistance
View Patent ↗A method for producing cucumber plants which are resistant to cucumber closteroviruses. The method comprises the steps of providing a Cucumis sativus plant which contains alleles that confer resistance to the closteroviruses defined by two QTLs, crossing said C. sativus plant with C. sativus culture breeding material, collecting the seeds resulting from said cross, regenerating the seeds into plants, evaluating the plants for resistance to the closteroviruses, and identifying and selecting resistant plants. Further, a resistant cucumber plant produced by the method as well as fruit or seed produced by said plant.
1. A cucumber elite line plant which is resistant to Beet pseudo-yellows virus (BPYV) or Cucurbit yellow stunting disorder virus (CYSDV) comprising DNA from Cucumis sativus accession PI 250147 conferring said virus resistance and DNA from Cucumis sativus culture breeding material
wherein the DNA within Cucumis sativus accession PI 250147 conferring said virus resistance is characterized by two quantitative trait loci, QTL1 and QTL2
wherein QTL1 is defined by (i) a marker of about 244 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, (ii) a marker of about 188 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2 and (iii) a marker of about 251 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:3, a cucumber genomic fragment and an MseI primer having SEQ ID NO:4, which are amplification products of alleles of Cucumis sativus accession PI 250147; and
wherein QTL2 is defined by (i) a marker of about 260 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, and (ii) a marker of about 107 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:6, which are amplification products of alleles of Cucumis sativus accession PI 250147.
2. Fruit obtained from a cucumber elite line plant which is resistant to Beet pseudo-yellows virus (BPYV) or Cucurbit yellow stunting disorder virus (CYSDV) comprising DNA from Cucumis sativus accession PI 250147 conferring said virus resistance and DNA from Cucumis sativus culture breeding material
wherein the DNA within Cucumis sativus accession PI 250147 conferring said virus resistance is characterized by two quantitative trait loci, QTL1 and QTL2
wherein QTL1 is defined by (i) a marker of about 244 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, (ii) a marker of about 188 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2 and (iii) a marker of about 251 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:3, a cucumber genomic fragment and an MseI primer having SEQ ID NO:4, which are amplification products of alleles of Cucumis sativus accession PI 250147; and
wherein QTL2 is defined by (i) a marker of about 260 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, and (ii) a marker of about 107 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:6, which are amplification products of alleles of Cucumis sativus accession PI 250147.
3. Seed obtained from a cucumber elite line plant which is resistant to Beet pseudo-yellows virus (BPYV) or Cucurbit yellow stunting disorder virus (CYSDV) comprising DNA from Cucumis sativus accession PI 250147 conferring said virus resistance and DNA from Cucumis sativus culture breeding material wherein the DNA within Cucumis sativus accession PI 250147 conferring said virus resistance is characterized by two quantitative trait loci, QTL1 and QTL2
wherein QTL1 is defined by (i) a marker of about 244 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, (ii) a marker of about 188 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2 and (iii) a marker of about 251 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:3, a cucumber genomic fragment and an MseI primer having SEQ ID NO:4, which are amplification products of alleles of Cucumis sativus accession PI 250147; and
wherein QTL2 is defined by (i) a marker of about 260 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, and (ii) a marker of about 107 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:6, which are amplification products of alleles of Cucumis sativus accession PI 250147.
4. A cucumber elite line plant which is resistant to Beet pseudo-yellows virus (BPYV) or Cucurbit yellow stunting disorder virus (CYSDV) comprising an introgression from Cucumis sativus accession PI 250147 conferring said virus resistance
wherein the introgression from Cucumis sativus accession PI 250147 conferring said virus resistance is characterized by
(i) a marker of about 244 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, a marker of about 188 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:1, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, and/or a marker of about 251 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:3, and a cucumber genomic fragment and an MseI primer having SEQ ID NO:4; and
(ii) a marker of about 260 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:2, and/or a marker of about 107 bp including from 5′ to 3′ of an EcoRI primer having SEQ ID NO:5, a cucumber genomic fragment and an MseI primer having SEQ ID NO:6 wherein the markers are amplification products of alleles of Cucumis sativus accession PI 250147.