Calibrating the Positions of a Rotating and Translating Two-Dimensional Scanner
Systems and methods are provided that comprise calibration techniques and associated systems that identify the two-dimensional position, or other alignment or positioning, of sample wells or other calibration objects located in a sample well plate, or other surface or area of interest. In some embodiments, calibration of the plate and/or positioning and/or alignment with respect to detection optics can be performed in multiple stages for two or more dimensions.
1 . A method of calibrating an alignment of a sample support, comprising:
receiving emission data from calibration objects of the sample support;
determining a center of a set of the calibration objects in a first dimension;
determining a set of translations of the calibration objects in a second dimension;
generating a calibrated alignment of the calibration objects based on the center and the determined set of translations.
2 . The method of claim 1 , wherein determining a center comprises determining a center corresponding to an angular position of an imaging element imaging the calibration objects.
3 . The method of claim 2 , wherein determining a center comprises summing or differencing a set of peaks in the emission data for the set of calibration objects in the second dimension at the same angular value.
4 . The method of claim 3 , wherein determining a center comprises identifying a highest summation of emission data in the second dimension, and associating the center with the angular value corresponding the highest summation of emission data.
5 . The method of claim 1 , wherein determining a set of translations comprises identifying a set of peaks in the emission data for a set of calibration objects in the second dimension.
6 . The method of claim 1 , further comprising generating a statistical measure of the separation distance between pairs of the set of calibration objects.
7 . A system for calibrating an alignment of a sample support and a detection device, comprising:
an input unit, the input unit being configured to receive emission data from calibration objects of the sample support; and
a processor unit, the processor unit communicating with the input unit and being configured to:
determine a center of a set of the calibration objects in a first dimension,
determine a set of translations of the calibration objects in a second dimension, and
generate a calibrated alignment of the calibration objects based on the center and the determined set of translations.
8 . The system of claim 7 , wherein determining a center comprises determining a center corresponding to an angular position of an imaging element imaging the calibration objects.
9 . The system of claim 7 , wherein determining a set of translations comprises identifying a set of peaks in the emission data for a set of calibration objects in the second dimension.
10 . The system of claim 7 , further comprising generating a statistical measure of the separation distance between pairs of the set of calibration objects.
11 . A calibrated representation of the position of calibration objects of a sample support, the calibrated representation being generated by a method comprising:
receiving emission data from calibration objects of the sample support;
determining a center of a set of the calibration objects in a first dimension;
determining a set of translations of the calibration objects in a second dimension;
generating a calibrated alignment of the calibration objects based on the center and the determined set of translations.
12 . The calibrated representation of claim 11 , wherein determining a center comprises determining a center corresponding to an angular position of an imaging element imaging the calibration objects.
13 . The calibrated representation of claim 11 , wherein determining a set of translations comprises identifying a set of peaks in the emission data for a set of calibration objects in the second dimension.
14 . The calibrated representation of claim 13 , wherein the set of translations in the second dimension comprises a set of linear offsets.
15 . The calibrated representation of claim 11 , wherein the method further comprises generating a statistical measure of the separation distance between pairs of the set of calibration objects.
16 . The calibrated representation of claim 11 , wherein the calibration objects comprise a set of sample wells.
17 . A computer-readable medium, the computer-readable medium being readable to execute a method of:
receiving emission data from calibration objects of the sample support;
determining a center of a set of the calibration objects in a first dimension;
determining a set of translations of the calibration objects in a second dimension;
generating a calibrated alignment of the calibration objects based on the center and the determined set of translations.
18 . The computer-readable medium of claim 17 , wherein determining a center comprises determining a center corresponding to an angular position of an imaging element imaging the calibration objects.
19 . The computer-readable medium of claim 17 , wherein the method further comprises generating a statistical measure of the separation distance between pairs of the set of calibration objects.
20 . The computer-readable medium of claim 17 , wherein the sample support comprises a plate.