AFM for simultaneous recognition of multiple factors

1. An atomic force microscope for detecting interactions between a probe and first and second sensed agents on a scanned surface, said microscope comprising:

a scanning probe having a tip that is sensitive to a property of said scanned surface;

first and second sensing agents tethered to the tip of the probe, said first and second sensing agents being sensitive to said first and second sensed agents, respectively, said first sensing agent being different from said second sensing agent; and

means for recording a displacement of said probe tip as a function of time, recording topographic images, and recording the spatial location of interactions between said probe and said first and second sensed agents on said scanned surface.

2. The atomic force microscope of claim 1 , wherein the two or more sensing agents are biomolecules.

3. The atomic force microscope of claim 2 , wherein the biomolecules are selected from the group consisting of: an antibody, a polypeptide, a nucleic acid, a lipid, a carbohydrate, or a ligand.

4. The atomic force microscope of claim 1 , wherein at least one of the two or more sensing agents is an aptamer.

5. The atomic force microscope of claim 1 , wherein at least one of the two or more sensing agents is an antibody.

6. The atomic force microscope of claim 1 , wherein at least one of the two or more sensing agents is a lectin.

7. The atomic force microscope of claim 1 , wherein said probe is adapted to oscillate with a mechanical Q factor equal to or less than 20.