Labeling reagents and methods of their use
View Patent ↗The present disclosure is directed to a reactive ester agent capable of conjugating a reporter molecule to a carrier molecule or solid support. The reactive ester agent has the general formula: wherein the variables are described throughout the application.
1. A compound having the following structure or a tautomer or salt thereof:
wherein,
R 1 is a halogen;
R 2 is a halogen; and
R 3 is a water solubilizing group.
2. The compound of claim 1 , wherein R 1 and R 2 are chloro.
3. The compound of claim 1 , wherein R 3 is —SO 3 ′.
4. The compound of claim 1 , wherein R 1 and R 2 are chloro and wherein R 3 is —SO 3 −.
5. The compound of claim 1 wherein the water solubilizing group comprises a polar substituent.
6. The compound of claim 1 wherein the water solubilizing group comprises a charged substituent that increases water solubility of a base molecule.
7. The compound of claim 6 wherein the water solubilizing group comprises an anionic substituent.
8. The compound of claim 6 wherein the water solubilizing group is appended directly to the base molecule.
9. The compound of claim 6 wherein the water solubilizing group is appended through a linker to the base molecule.
10. The compound of claim 1 wherein the water solubilizing group comprises a carboxyl group, sulphonic acid, hydroxyl group, substituted azenyl group, a polyoxyalkylene, phosphate group, or a bisphosphonate group.
11. The compound of claim 10 wherein the water solubilizing group comprises a polyoxyalkylene and the polyoxyalkylene is poly (ethylene glycol).
12. The compound of claim 1 wherein the water solubilizing group comprises a substituent that introduces an additional net charge into the molecule.