PRODUCTION OF L-LYSINE AND L-LYSINE-CONTAINING FEED ADDITIVES
The invention relates to a process for producing L-lysine or L-lysine-containing feed additives by fermentation, which comprises a) expressing a polynucleotide coding for polypeptide having LL-diaminopimelate aminotransferase activity in a bacterium excreting L-lysine, and b) fermenting the resultant bacterium in a medium under suitable conditions and allowing the resultant L-lysine to accumulate in the fermentation broth.
1 - 27 . (canceled)
28 . A process for producing L-lysine or L-lysine-containing feed additives by fermentation, comprising:
a) expressing a polynucleotide coding for a polypeptide having LL-diaminopimelate aminotransferase activity in an L-lysine excreting bacterium; and
b) fermenting the resultant bacterium in a medium under suitable conditions and allowing the resultant L-lysine excreted by the bacterium to accumulate in the fermentation broth.
29 . The process of claim 28 , wherein the polynucleotide coding for a polypeptide having LL-diaminopimelate aminotransferase activity is a polynucleotide from Arabidopsis thaliana, Synechococcus sp., Oryza sativa or Vitis vinifera.
30 . The process of claim 28 , wherein the bacterium is a bacterium selected from the group coryneform bacteria, the family Enterobacteriaceae and the genus Bacillus.
31 . The process of claim 30 , wherein the coryneform bacterium is a bacterium of the genus Corynebacterium.
32 . The process of claim 31 , wherein the bacterium of the genus Corynebacterium is a bacterium selected from the group Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium callunae, Corynebacterium thermoaminogenes and Corynebacterium ammoniagenes.
33 . The process of claim 32 , wherein the bacterium is Corynebacterium glutamicum.
34 . The process of claim 30 , wherein the bacterium of the family Enterobacteriaceae is a bacterium of the genus Escherichia.
35 . The process of claim 34 , wherein the bacterium of the genus Escherichia is Escherichia coli.
36 . The process of claim 30 , characterized in that the bacterium of the genus Bacillus is Bacillus subtilis or Bacillus methanolicus.
37 . The process of claim 28 , wherein said bacterium excretes at least 0.25 g/l L lysine in maximally 120 hours.
38 . The process of claim 28 , wherein bacterium possesses a gene coding for a feedback resistant aspartate kinase.
39 . The process of claim 38 , wherein the polypeptide has an amino acid sequence which is at least 70% identical to the amino acid sequences selected from the group: SEQ ID NO: 2 SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10.
40 . The process of claim 39 , wherein the polypeptide has an amino acid sequence which is identical to an amino acid sequence selected from the group: SEQ ID NO: 2 SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, and SEQ ID NO: 10.
41 . The process of claim 28 , wherein the bacterium is fermented in a fed-batch process.
42 . The process of claim 28 , wherein the bacterium is fed in a repeated fed-batch process.
43 . The process of claim 28 , wherein the bacterium is fermented in a continuous process.
44 . The process of claim 28 , wherein the L-lysine excreted by the bacterium is collected.
45 . The process of claim 28 , wherein the L-lysine excreted by the bacterium is obtained together with components of the fermentation broth.
46 . The process of claim 28 , wherein the L-lysine excreted by the bacterium is isolated and purified.
47 . An L-lysine-excreting bacterium which has been transformed by a polynucleotide coding for a polypeptide having LL-diaminopimelate aminotransferase activity and, compared with a non-transformed bacterium, exhibits increased excretion of L-lysine.
48 . The L-lysine-excreting bacterium of claim 47 , wherein the polypeptide having LL-diaminopimelate aminotransferase activity is a polynucleotide of Arabidopsis thaliana, Synechococcus sp., Oryza sativa or Vitis vinifera.
49 . The L-lysine-excreting bacterium of claim 47 , wherein the bacterium is a coryneform bacterium.
50 . The L-lysine-excreting bacterium of claim 49 , wherein the bacterium is a coryneform bacterium of the genus Corynebacterium.
51 . The L-lysine-excreting bacterium of claim 50 , wherein the bacterium of the genus Corynebacterium is Corynebacterium glutamicum.
52 . The L-lysine-excreting bacterium of claim 47 , wherein the bacterium is a bacterium of the family Enterobacteriaceae.
53 . The L-lysine-excreting bacterium of claim 52 , wherein the bacterium of the family Enterobacteriaceae is of the genus Escherichia.
54 . The L-lysine-excreting bacterium of claim 53 , wherein the bacterium of the genus Escherichia is Escherichia coli.