Functionalized fluorescent nanocrystals, and methods for their preparation and use
Functionalized fluorescent nanocrystal compositions and methods for making and using these compositions are disclosed. The compositions are fluorescent nanocrystals coated with at least one material. The coating material has chemical compounds or ligands with functional groups or moieties with conjugated electrons and moieties for imparting solubility to coated fluorescent nanocrystals in aqueous solutions. The coating material provides for functionalized fluorescent nanocrystal compositions which are water soluble, chemically stable, and emit light with a high quantum yield and/or luminescence efficiency when excited with light. The coating material may also have chemical compounds or ligands with moieties for bonding to target molecules and cells as well as moieties for cross-linking the coating. In the presence of reagents suitable for reacting to form capping layers, the compounds in the coating may form a capping layer on the fluorescent nanocrystal with the coating compounds operably bonded to the capping layer.
1 . A functionalized fluorescent nanocrystal comprising:
a fluorescent nanocrystal; and
a coating material comprising an electron rich heteroaromatic structure.
2 . The functionalized fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal comprises a core and a shell.
3 . The functionalized fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal comprises a CdS, CdSe, or CdTe core.
4 . The functionalized fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal comprises a core and a ZnS, ZnSe, CdS, or CdSe shell.
5 . The functionalized fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal comprises a CdS or CdSe core, and a ZnS or ZnSe shell.
6 . The functionalized fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal comprises a CdSe core, and a ZnS shell.
7 . The fluorescent nanocrystal of claim 1 , wherein the fluorescent nanocrystal is a doped metal oxide nanocrystal.
8 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is histidine, carnosine, anserine, baleine, homocarnosine, histidylphenylalanine, cyclo-histidylphenylalanine, 5-amino-4-imidazolecarboxamide, histidylleucine, 2-mercaptoimidazole, boc-histidine hydrazide, histidinol, 1-methylhistidine, 3-methylhistidine, imidazolysine, an imidazole-containing ornithine, 5-methylimidazolone, an imidazole-containing alanine, beta-(2-imidazolyl)-L(alpha) alanine, carcinine, histamine, or glycl histidine.
9 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is glycl histidine.
10 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is carnosine.
11 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is thiazole, a thiazole derivative, oxazole, an oxazole derivative, pyrrole, a pyrrole derivative, a doped poly-pyrrole oligomer, an undoped poly-pyrrole oligomer, thiophene, a thiophene derivative, doped poly-thiophene, undoped poly-thiophene, furan, a furan derivative, pyridine, a pyridine derivative, pyrimidine, a pyrimidine derivative, pyrazine, a pyrazine derivative, triazine, a triazine derivative, triazole, a triazole derivative, phthalocyanine, a phthalocyanine derivative, porphyrin, and a porphyrin derivative.
12 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is a conjugated polymer.
13 . The functionalized fluorescent nanocrystal of claim 1 , wherein the coating material is polyaniline (PAn), poly(N-vinylcarbazole) (PVCZ), polyacetylene, or polypyrrole.
14 . The functionalized fluorescent nanocrystal of claim 1 , characterized as emitting light with a quantum yield of greater than about 10%.
15 . The functionalized fluorescent nanocrystal of claim 1 , characterized as emitting light with a quantum yield of greater than about 30%.
16 . The functionalized fluorescent nanocrystal of claim 1 , characterized as emitting light with a quantum yield of greater than about 50%.
17 . The functionalized fluorescent nanocrystal of claim 1 , characterized as emitting light with a quantum yield of greater than about 70%.
18 . The functionalized fluorescent nanocrystal of claim 1 , characterized as being soluble in a fluid having a surface energy of greater than 35 dynes/cm.
19 . The functionalized fluorescent nanocrystal of claim 1 , characterized as being soluble in an aqueous solution.
20 . The functionalized fluorescent nanocrystal of claim 1 , further comprising a polynucleotide attached to the coating material.
21 . The functionalized fluorescent nanocrystal of claim 1 , further comprising a polynucleotide covalently attached to the coating material.
22 . The functionalized fluorescent nanocrystal of claim 1 , further comprising a biomolecule attached to the coating material, wherein the biomolecule is biotin, avidin, ConA, lectin, or IgG.
23 . The functionalized fluorescent nanocrystal composition of claim 1 , further comprising avidin attached to the coating material.
24 . The functionalized fluorescent nanocrystal composition of claim 1 , wherein the coating material is crosslinked with tris(hydroxy methyl) phosphine or beta-(tris(hydroxymethyl)phosphino)propionic acid.
25 . A functionalized fluorescent nanocrystal comprising:
a fluorescent nanocrystal; and
a coating material comprising a pyrrole containing compound.
26 . The functionalized fluorescent nanocrystal of claim 25 , wherein the pyrrole containing compound is a porphyrin or coproporphyrin I dihydrochloride.
27 . A method for preparing a functionalized fluorescent nanocrystal, the method comprising:
providing a fluorescent nanocrystal; and
contacting the fluorescent nanocrystal with a coating material comprising an electron rich heteroaromatic structure, to prepare a functionalized fluorescent nanocrystal.
28 . The method of claim 27 , wherein the fluorescent nanocrystal comprises a CdSe core and a ZnS shell.
29 . The method of claim 27 , wherein the fluorescent nanocrystal is a doped metal oxide nanocrystal.
30 . A method for detecting a target species, the method comprising:
providing a sample suspected of containing a target species;
contacting the target species and at least one functionalized fluorescent nanocrystal to form a complex;
exciting the complex; and
detecting radiation emitted from the complex;
wherein the functionalized fluorescent nanocrystal comprises a fluorescent nanocrystal and a coating material comprising an electron rich heteroaromatic structure.
31 . The method of claim 30 , wherein the detecting step further comprises detecting the intensity of radiation emitted from the complex in the perpendicular and parallel directions.
32 . A method for separating materials, the method comprising:
providing a mixture of a fluid and at least one functionalized fluorescent nanocrystal, wherein the functionalized fluorescent nanocrystal comprises a doped metal oxide fluorescent nanocrystal and a coating material comprising an electron rich heteroaromatic structure;
exciting the functionalized fluorescent nanocrystal such that it has a magnetic moment; and
exposing the mixture to a magnetic field to separate the functionalized fluorescent nanocrystal.
33 . The method of claim 32 , wherein the doped metal oxide fluorescent nanocrystal further comprises an affinity molecule operably bound to the functionalized fluorescent nanocrystal.
34 . The method of claim 32 , wherein:
the doped metal oxide fluorescent nanocrystal further comprises an affinity molecule operably bound to the functionalized fluorescent nanocrystal; and
the affinity molecule is an antibody, a protein, a peptide, an antibody, an antibody fragment, or a nucleic acid.