Methods for Diagnosing Cancer Using Samples Collected From A Central Vein Location or an Arterial Location
The invention encompasses methods for selectively enriching in rare particles from blood samples harvested from the vein jugular vein the femoral vein, the subclavian vein, or an artery. These rare particles can be circulating tumor cells, circulating stem cells, or fragments thereof. Blood samples harvested from different sources can contain higher or lower concentrations of rare particles. The rare particles can be enriched by applying the blood samples to a microfluidic device with a two dimensional array of obstacles.
1 . A method for selectively concentrating one or more rare particles from an organism comprising: applying a blood sample taken from a jugular vein, a femoral vein, a subclavian vein, an artery or a heart to a device that selectively enriches the one or more rare particles.
2 . The method of claim 1 , wherein the artery is a radial artery, an ulnar artery, a brachial artery, a femoral artery, a carotid artery, a subclavian artery or a brachiocephalic artery.
3 . The method of claim 1 , wherein the organism is a human.
4 . The method of claim 1 , wherein the one or more rare particles comprise one or more circulating tumor cells, circulating stem cells, or fragments thereof.
5 . The method of claim 1 , wherein device is a flow-through device.
6 . The method of claim 3 , wherein said device selectively retains said rare particles.
7 . The method of claim 1 , wherein said device is a microfluidic device.
8 . The method of claim 7 , wherein the microfluidic device comprises a two-dimensional array of obstacles.
9 . The method of claim 7 , wherein the microfluidic device is functionalized with binding moieties.
10 . The method of claim 9 , wherein the binding moieties specifically binds EPCAM, E-Cadherin, Mucin-1, Cytokeratin, EGFR, LAR, CD34 or folate receptor.
11 . A method for diagnosing, prognosing, or theranosis of cancer in a subject, said method comprising the steps of:
a) enriching one or more circulating tumor cells or fragments thereof from a blood sample taken from a jugular vein, a femoral vein, a subclavian vein, an artery or a heart and
b) diagnosing, prognosing, or theranosing based on analysis of said enriched one or more circulating tumor cells or fragments thereof.
12 . The method of claim 11 , wherein the artery is a radial artery, an ulnar artery, a brachial artery, a femoral artery, a carotid artery, a subclavian artery or a brachiocephalic artery.
13 . The method of claim 11 , wherein enriching step comprises applying the blood sample to a microfluidic device that selectively captures the one or more circulating tumor cells or fragments thereof.
14 . The method of claim 13 , wherein the selective capture occurs based on affinity, size, shape or deformability.
15 . The method of claim 13 , wherein the microfluidic device is covered by at least one binding moiety that selectively binds the one or more circulating tumor cells or fragments thereof.
16 . A method of claim 11 , further comprising enumerating the one or more circulating tumor cells or fragments thereof.