Culture medium for gingival fibroblasts
View Patent ↗The present invention relates to a gingival fibroblast culture medium free of animal serum, comprising an animal cell culture medium, free of animal serum, to which is added: from 0.1 ng/ml to 100 ng/ml bFGF, and/or from 1 μg/ml to 50 μg/ml insulin.
1. A method for the culture of gingival fibroblasts which comprises growing gingival fibroblasts in a gingival fibroblast culture medium, wherein said medium comprises a culture medium for animal or human cells, free of animal serum to which is added: from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μg/ml to 50 μg/ml insulin.
2. A kit for cultivating gingival fibroblasts, comprising:
a gingival fibroblast culture medium comprising a culture medium for animal or human cells, free of animal serum to which is added: from 0.1 ng/ml to 100 ng/ml bFGF and from 1 μg/ml to 50 μg/ml insulin and
platelet lysate.
3. The method according to claim 1 , wherein bFGF is added at 1 ng/ml.
4. The method according to claim 1 , wherein insulin is added at 5 μg/ml.
5. The method according to claim 1 , wherein the gingival fibroblast culture medium free of animal serum comprises bFGF at 1 ng/ml and insulin at 5 μg/ml.
6. The method according to claim 1 , wherein the gingival fibroblast culture medium free of animal serum further comprises platelet lysate.