Polypeptides having alpha-glucosidase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
1. An isolated polynucleotide comprising a nucleotide sequence which encodes a polypeptide having alpha-glucosidase activity, wherein the isolated polynucleotide is selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having alpha-glucosidase activity comprising an amino acid sequence which has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(b) a polynucleotide encoding a polypeptide having alpha-glucosidase activity comprising a nucleotide sequence which has at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; and
(c) a polynucleotide encoding a polypeptide having alpha-glucosidase activity comprising a nucleotide sequence which hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
2. The polynucleotide of claim 1 , wherein the polypeptide having alpha-glucosidase activity comprises an amino acid sequence which has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2.
3. The polynucleotide of claim 2 , wherein the polypeptide having alpha-glucosidase activity comprises an amino acid sequence which has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.
4. The polynucleotide of claim 1 , wherein the polypeptide having alpha-glucosidase activity comprises or consists of the amino acid sequence of SEQ ID NO: 2, or a fragment thereof having alpha-glucosidase activity.
5. The polynucleotide of claim 1 , which comprises a nucleotide sequence which has at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
6. The polynucleotide of claim 5 , which comprises a nucleotide sequence which has at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.
7. The polynucleotide of claim 1 , which comprises or consists of the nucleotide sequence of SEQ ID NO: 1, or a subsequence thereof encoding a polypeptide fragment having alpha-glucosidase activity.
8. The polynucleotide of claim 1 , which comprises a nucleotide sequence which hybridizes under at least high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1, or (iii) a full-length complementary strand of (i) or (ii).
9. The polynucleotide of claim 1 , which is contained in plasmid pFD11F2 which is contained in E. coli NRRL B-30753.
10. The isolated polynucleotide of claim 1 , wherein the mature polypeptide is amino acids 21 to 960 of SEQ ID NO: 2.
11. The isolated polynucleotide of claim 1 , wherein the mature polypeptide coding sequence is nucleotides 61 to 2880 of SEQ ID NO: 1.
12. A nucleic acid construct comprising the polynucleotide of claim 1 operably linked to one or more control sequences that direct the production of the polypeptide having alpha-glucosidase activity in an expression host.
13. A recombinant expression vector comprising the nucleic acid construct of claim 12 .
14. An isolated transformed host cell comprising the nucleic acid construct of claim 12 .
15. A method for producing a polypeptide having alpha-glucosidase activity, comprising (a) cultivating a host cell comprising the nucleic acid construct of claim 12 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.