Tagged Polyfunctional Reagents Capable of Reversibly Binding Target Substances in a pH-dependent Manner
Polyfunctional reagents are disclosed that are capable of reversibly binding to target substances, for example nucleic acid, proteins, polypeptides, cells, cell components, microorganisms or viruses, for use in purifying or otherwise manipulating them. The reagents comprise a tagging group for manipulating and/or detecting the target substance when bound to the polyfunctional reagent. The polyfunctional reagents work by binding the target substance at a first pH and then releasing it at a second pH, usually higher than the first. Examples of tagging groups include tagging group members of a specific binding pair which is capable of binding to a specific binding partner and/or a label.
1 . A soluble polyfunctional reagent which is capable at a first pH of binding to a target substance and is capable at a second pH of releasing the target substance, wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent.
2 . The polyfunctional reagent of claim 1 , wherein the reagent is capable of reversibly binding to the target substance at a first pH at which the reagent is positively charged and releasing the nucleic acid at a second, higher pH at which the reagent is less positive, neutral or negatively charged.
3 . The polyfunctional reagent of claim 2 , wherein the reagent has positively ionisable groups having a pKa between 4.5 and 8.5.
4 . The polyfunctional reagent of claim 2 , wherein the first pH at which the target substance binds is no less than pH 3.0 and the second pH at which the target substance is released is between pH 7.0 and 9.0.
5 . The polyfunctional reagent of claim 4 , wherein the target substance is nucleic acid.
6 . The polyfunctional reagent of claim 5 , wherein the nucleic acid is single or double stranded DNA, RNA or oligonucleotides.
7 . The polyfunctional reagent of claim 6 , wherein the nucleic acid is non-genomic nucleic acid, cellular vector DNA or RNA, self-replicating satellite nucleic acids or plasmid DNA, genomic nucleic acid, host cell chromosomes or ribosomal RNA.
8 . The polyfunctional reagent of claim 1 , wherein the reagent is capable of reversibly binding to the target substance at a first pH at which the reagent is negatively charged and releasing the nucleic acid at a second, lower pH at which the reagent is less negative, neutral or positively charged.
9 . The polyfunctional reagent of claim 8 , wherein the reagent has negatively ionisable groups having a pKa between 3.0 and 7.0.
10 . The polyfunctional reagent of claim 8 , wherein the target substance is a protein, a polypeptide, a cell or a component of a cell, a lipid, a carbohydrate, a virus or a microorganism.
11 . The polyfunctional reagent of claim 1 , wherein reagent releases the target substance:
(a) substantially in the absence of strong mineral base; and/or
(b) at a temperature which is less than 70° C.; and/or
(c) at an ionic strength which is below 100 mM.
12 . The polyfunctional reagent of claim 1 , wherein the reagent is a polyhydroxylated amine, a polymerised biological buffer, or a polymerised amino acids.
13 . The polyfunctional reagent of claim 12 , wherein the reagent is poly Bis-Tris, poly-iris, polyhistidine, a polyhydroxylated amine, a chitosan, or a triethanolamine.
14 . The polyfunctional reagent of claim 1 , wherein the reagent comprises a plurality of tagging groups.
15 . The polyfunctional reagent of claim 1 , wherein the tagging group is a member of a specific binding pair which is capable of binding to a specific binding partner and/or the tagging group is a label.
16 . The polyfunctional reagent of claim 15 , wherein the specific binding pair is an antibody and an antigen, a label and an antibody capable of binding the label, biotin and avidin or streptavidin, a ligand and a receptor, a lectin and a carbohydrate, an enzyme and a cofactor or substrate, a bacteriophage binding to microbial cell walls or a component thereof, cells binding via receptors or antibodies or lectins, appositely charged ionic groups, redox/electrochemical groups, a chelating group and its binding partner, two hydrophobic substances that are capable of binding to a each other in an aqueous system, a nucleic acid intercalating group and nucleic acid, or a nucleic acid molecule capable of hybridizing to a complementary nucleic acid sequence.
17 . The polyfunctional reagent of claim 15 or claim 16 , wherein the label is a fluorescent label.
18 . The polyfunctional reagent of claim 15 , wherein the specific binding pair is biotin and avidin, biotin and streptavidin, fluorescein isothiocyanate (FITC) and an anti-FITC antibody, an antidigoxygenin antibody and digoxygenin, maltose binding protein and maltose, glutathione binding to GST, an ethidium bromide or Cy3 intercalating group and nucleic acid, Ni 2+ ion and polyhistidine, or Concanavalin A binding to sugar residues.
19 . A kit comprising a polyfunctional reagent of claim 1 , optionally in combination with a solid phase on which the polyfunctional reagent is bound or is capable of binding.
20 . The kit of claim 19 , wherein the solid phase is a magnetisable material, a tube, a well, a tip, a probe, a pipette, a membrane, a filter, a bead, a particle, a sheet, a slide, a plug.
21 . The kit of claim 20 , wherein the solid phase is magnetic or paramagnetic beads.
22 . The kit of claim 19 , wherein the solid phase is formed from glass, silica, plastic, a mineral, a carbohydrate, paper, a cellulose or a combination thereof.
23 . A method employing a soluble polyfunctional reagent of claim 1 , wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent, the method comprising:
contacting a sample containing a target substance with the polyfunctional reagent under conditions such that the target substance binds to the polyfunctional reagent; and
manipulating or detecting the reagent and/or the bound target substance by employing the tagging group.
24 . The method of claim 23 , wherein manipulating the reagent using the tagging group comprises contacting the polyfunctional reagent binding to the target substance with a solid phase on which the binding partner of the specific binding member has been immobilized so that the specific binding pair bind and separating the polyfunctionalized reagent and any bound target substance.
25 . The method of claim 24 , wherein the polyfunctional reagent comprises a detectable label that is a specific binding member capable of binding to a binding partner immobilized on a solid phase.
26 . The method of claim 25 , wherein the polyfunctional reagent comprises a fluorescent label and the binding partner comprises antibodies capable of binding to the fluorescent label immobilized on the solid phase.
27 . The method of claim 26 , wherein manipulating the reagent using the tagging group comprises separating the target substance binding to the polyfunctional reagent by means of a fluorescent activated cell sorter.
28 . A method employing a soluble polyfunctional reagent of claim 1 , wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent, the method comprising:
contacting the polyfunctional reagent with a solid phase having immobilized thereon a member of a specific binding pair capable of binding to the tagging group so that the polyfunctional reagent binds to the solid phase;
contacting a sample containing a target substance with the polyfunctional reagent under conditions where the target substance binds to the polyfunctional reagent.
29 . The method of claim 23 , wherein the target substance is nucleic acid.
30 . The method of claim 23 , wherein the solid phase is a magnetisable material, a tube, a well, a tip, a probe, a pipette, a membrane, a filter, a bead, a particle, a sheet, a slide, a plug.
31 . The method of claim 30 , wherein the solid phase is magnetic or paramagnetic beads.
32 . The method of claim 23 , wherein the solid phase is formed from glass, silica, plastic, a mineral, a carbohydrate, paper, a cellulose or a combination thereof.
33 . A polyfunctional reagent which is capable at a first pH at which the reagent is positively charged of binding to a target substance and is capable at a second, higher pH at which the reagent is less positive, neutral or negatively charged of releasing the target substance, wherein the reagent further comprises a tagging group for manipulating or detecting the target substance when bound to the polyfunctional reagent and wherein the reagent has positively ionisable groups having a pKa between 4.5 and 8.5.