Liquid Handling System and Methods for Mixing and Delivering Liquid Reagents
A liquid storage apparatus provides a safe and easy to use device for efficiently managing liquid reagents used in a variety of laboratory equipment. The liquid storage apparatus helps reduce the likelihood of accidents, allows for flexibility of experimental design, and helps maximize the use of chemical regents to prevent waste. The apparatus includes a plurality of containers with a pierceable septum interface at each end. The apparatus also includes a lower array of needles with each of the lower needles in the lower array of needles arranged to penetrate the bottom pierceable septum of a different one of the containers. The apparatus further includes a piercing device arranged to penetrate the top pierceable septum of a different one of the containers. Each of the piercing devices include a passageway so gas can flow into the pierced container.
1 . A liquid storage apparatus for use in connection with microfluidic volume analyzing equipment, comprising:
a plurality of containers, each container comprising a top pierceable septum and a bottom pierceable septum;
a lower array of needles, each of the lower needles for penetrating the bottom pierceable septum of a different one of the containers; and
a piercing device for penetrating the top pierceable septum, each piercing device including a passage through which a gas flows into the container.
2 . The liquid storage apparatus of claim 1 , wherein the piercing device further comprises:
a housing defining an internal region for receiving a container having a pierceable septum, the housing having one or more slots to allow expansion of the housing;
a septum piercing element affixed to the housing, the septum piercing element having a pointed tip and defining a passageway; and
a protrusion for selectively engaging an outer surface of the container,
wherein the protrusion positions and retains the container in the housing in an un-actuated position such that the septum piercing element is not in contact with the septum prior to manipulation
3 . The liquid storage apparatus of claim 2 , wherein the piercing device further comprises:
a finger for selectively securing the container in the housing.
4 . The liquid storage apparatus of claim 3 , wherein the finger engages the outer surface of the container and secures the container in the housing in an actuated position such that the septum piercing element pierces the septum of the container after manipulation.
5 . The liquid storage apparatus of claim 2 , wherein the housing is manipulated from the un-actuated position to an actuated position such that the piecing element in the actuated position pierces the septum.
6 . A piercing device comprising:
a housing defining an internal region for receiving a container having a pierceable septum, the housing having one or more slots to allow expansion of the housing;
a septum piercing element affixed to the housing, the septum piercing element having a pointed tip and defining a passageway; and
a protrusion for selectively engaging an outer surface of the container,
wherein the protrusion positions and retains the container in the housing in an un-actuated position such that the septum piercing element is not in contact with the septum prior to manipulation
7 . The piercing device of claim 6 , furthering comprising a finger for selectively securing the container in the housing.
8 . The piercing device of claim 7 , wherein the finger engages the outer surface of the container and secures the container in the housing in an actuated position such that the septum piercing element pierces the septum of the container after manipulation.
9 . The piercing device of claim 6 , wherein the housing is manipulated from the un-actuated position to an actuated position such that the piecing element in the actuated position pierces the septum.
10 . The piercing device of claim 9 , wherein the passageway is in fluid communication with the container.