Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.
1. An isolated nucleic acid comprising:
(a) the nucleotide sequence set forth in SEQ ID NO:1;
(b) a nucleotide sequence that hybridizes under highly stringent conditions to the complement of the nucleotide sequence set forth in SEQ ID NO:1; or
(c) the complement of the nucleotide sequence set forth in SEQ ID NO:1.
2. A vector comprising the nucleic acid sequence of claim 1 .
3. A cultured cell comprising the vector of claim 2 .
4. The cultured cell of claim 3 , wherein said cell is a fungal cell.
5. The cultured cell of claim 4 , wherein said fungal cell is Yarrowia lipolytica or Arxula adeninivorans.
6. An isolated nucleic acid comprising a nucleotide sequence that is at least 90% identical to SEQ ID NO:1.
7. A vector comprising the nucleic acid of claim 6 .
8. A cultured cell comprising the vector of claim 7 .
9. The cultured cell of claim 8 , wherein said cell is a fungal cell.
10. The cultured cell of claim 9 , wherein said fungal cell is Yarrowia lipolytica or Arxula adeninivorans.
11. The nucleic acid of claim 6 , wherein said nucleotide sequence is at least 95% identical to SEQ ID NO:1.
12. An isolated nucleic acid comprising:
(a) the nucleotide sequence set forth in SEQ ID NO:2;
(b) a nucleotide sequence that hybridizes under highly stringent conditions to the complement of SEQ ID NO:2; or
(c) the complement of the nucleotide sequence set forth in SEQ ID NO:2.
13. A vector comprising the nucleic acid of claim 12 .
14. A cultured cell comprising the vector of claim 13 .
15. The cultured cell of claim 14 , wherein said cell is a fungal cell.
16. The cultured cell of claim 15 , wherein said fungal cell is Yarrowia lipolytica or Arxula adeninivorans.
17. An isolated nucleic acid comprising a nucleotide sequence that is at least 90% identical to SEQ ID NO:2.
18. A vector comprising the nucleic acid of claim 17 .
19. A cultured cell comprising the vector of claim 18 .
20. The cultured cell of claim 19 , wherein said cell is a fungal cell.
21. The cultured cell of claim 20 , wherein said fungal cell is Yarrowia lipolytica or Arxula adeninivorans.
22. The isolated nucleic of claim 17 , wherein said nucleotide sequence is at least 95% identical to SEQ ID NO:2.