Implantable cartilaginous tissue repair device
A cartilaginous tissue repair device with a biocompatible, bioresorbable three-dimensional silk or other fiber lay and a biocompatible, bioresorbable substantially porous silk-based or other hydrogel partially or substantially filling the interstices of the fiber lay; with or without an integral means of firmly anchoring the device to a patient's bone.
1. An implantable cartilaginous tissue repair device comprising:
a biocompatible and at least partially bioresorbable three-dimensional fibre lay comprising silk fibres
at least partially infiltrated by
a biocompatible and at least partially bioresorbable, substantially porous hydrogel comprising silk protein cross-linked by hydrogen bonds to form a beta sheet configuration, the hydrogel being cross-linked to the fibre lay,
wherein the hydrogel is non-flowable and has an open pore structure,
whereby, in use, cells can be introduced into and contained within at least some pores of the open pore structure of the hydrogel.
2. An implantable cartilaginous tissue repair device according to claim 1 in which said three-dimensional fibre lay is substantially biomimetic of the fibre pattern of a cartilaginous tissue which is to be repaired.
3. An implantable cartilaginous tissue repair device according to claim 1 in which the three-dimensional fibre lay comprises fibres selected from the group consisting of: natural mulberry-silk fibres, natural wild-silk fibres, spider-silk fibres, fibres of a recombinant protein based substantially on the proteins of mulberry silk, fibres of a recombinant protein based substantially on the proteins of wild silk, and fibres of a recombinant protein based substantially on the proteins of spider silk.
4. An implantable cartilaginous tissue repair device according to claim 1 in which said substantially porous hydrogel comprises at least one component selected from the group consisting of: regenerated silk fibroin from mulberry silk and regenerated silk fibroin from wild silk.
5. An implantable cartilaginous tissue repair device according to claim 1 in which the average pore diameter is between 50 μm and 300 μm.
6. An implantable cartilaginous tissue repair device according to claim 1 further comprises:
one or more covalently bound growth factors selected from the group consisting of: beta-fibroflast growth factor, transforming growth factor-beta 1, growth differentiation factor-5, insulin-like growth factor, basic fibroblast growth factor, cartilage tissue growth factor and osteogenic protein-I,
whereby, the binding and/or differentiation of mesenchymal or stem cells is stimulated in order to either form cartilage or to stimulate protoglycan secretion.
7. An implantable cartilaginous tissue repair device according to claim 1 further comprises:
an integral attachment means for attaching said three-dimensional fibre lay and said porous hydrogel to bone.
8. An implantable cartilaginous tissue repair device according to claim 7 in which said integral attachment means comprises at least one mineralised surface layer, extension or flange.
9. An implantable cartilaginous tissue repair device according to claim 8 in which said at least one mineralised surface layer, extension or flange comprises hydroxyapatite.
10. An implantable cartilaginous tissue repair device according to claim 7 in which said integral attachment means further comprises:
one or more covalently bound growth factors selected from the group consisting of: a bone morphogenetic protein, a transforming growth factor-beta, an epidermal growth factor, an insulin-like growth factor, growth/differentiation factor-10 and Runx2 (Cbfa1/AML3) transcription factor
whereby, the recruitment, binding and/or differentiation of mesenchymal or stem cells is stimulated in order to secrete normal bone.
11. An implantable cartilaginous tissue repair device according to claim 1 for total or partial replacement or augmentation of articular cartilage in which the orientation of the fibres in said three-dimensional fibre lay is substantially similar to the fibre orientation in a section of articular cartilage which is to be repaired.
12. An implantable cartilaginous tissue repair device according to claim 1 in which said three-dimensional fibre lay is formed as an arching arcade structure comprising:
at least one substantially flat base layer;
at least one substantially flat top articular surface layer which is substantially parallel to said base layer; and
a plurality of looped fibres which:
are stitched through said base layer;
run substantially perpendicular to said base layer and to said top articular surface layer; and
are held in place at the base layer and/or at the top articular surface layer by tying.
13. An implantable cartilaginous tissue repair device according to claim 1 in which the orientation of the fibres in said three-dimensional fibre lay is substantially similar to fibre orientation in an anatomical temporomandibular meniscus.
14. An implantable cartilaginous tissue repair device according to claim 1 in which said substantially porous hydrogel has an interconnecting pore structure.
15. An implantable cartilaginous tissue repair device according to claim 1 wherein the hydrogel is stiff.
16. An implantable cartilaginous tissue repair device according to claim 1 wherein not all of the pores contain cells.
17. An implantable tissue repair device comprising:
a biocompatible and at least partially bioresorbable fibre lay comprising silk fibres at least partially infiltrated by:
a biocompatible and at least partially bioresorbable porous hydrogel comprising silk protein cross-linked by hydrogen bonds to form a beta sheet configuration, the hydrogel being cross-linked to the fibre lay, wherein the hydrogel is non-flowable and has an open pore structure, whereby in use cells can be introduced into and contained within at least some pores of the open pore structure of the hydrogel, and
an integral attachment means for attaching said fibre lay and said porous hydrogel to bone comprising at least one mineralised surface.
18. A method for total or partial replacement or augmentation of an intervertebral disc, the method comprising implanting an implantable cartilaginous tissue repair device according to claim 1 as a total or partial replacement or augmentation of an intervertebral disc, which device is substantially shaped like an anatomical intervertebral disc.
19. A method for total or partial replacement or augmentation of a knee meniscus, the method comprising implanting an implantable cartilaginous tissue repair device according to claim 1 for total or partial replacement or augmentation of a knee meniscus, which device is substantially shaped like an anatomical knee meniscus.
20. A method according to claim 19 in which said three-dimensional fibre lay comprises a plurality of fibres arranged substantially circumferentially and a plurality of fibres arranged substantially radially.