System for the detection and enumeration of suspect target cells in a mixed cell population
The invention relates generally to the field of identification of DNA sequences, genes or chromosomes. Methods and composition to obtain Unique Sequence DNA probes are provided. Composition comprises of any double stranded DNA containing Unique Sequences from which the repetitive sequences are eliminated according to the method described in this invention. The invention also relates to the preservation of cells that have been identified after immunomagnetic selection and fluorescent labeling in order to further interrogate the cells of interest. Furthermore the invention relates to genetic analysis of cells that have been identified after immunomagnetic selection and fluorescent labeling.
1. A system for the detection and enumeration of suspect target cells in a mixed cell population, comprising:
a. means for obtaining a biological specimen from a test subject, said specimen comprising a mixed cell population suspected of containing target cells;
b. means for isolating a subpopulation of suspect said target cells by immunomagnetic enrichment using colloidal magnetic particles;
c. means for identifying suspect target cells by a phenotypic profile analysis means wherein a magnetic holding force holds said target cells for imaging;
d. means for preparing suspect target cells identified by the means of step c for a genotypic profile analysis means wherein shear forces to replace preparatory buffers on the target cells are less than the magnetic holding forces required to maintain the target cells for imaging; and
e. means for confirming suspect target cells by said genotypic profile wherein said individual suspect target cell contains both phenotypic and genotypic characteristics.
2. The system of claim 1 wherein said means for obtaining a biological specimen includes fixation of said specimen.
3. The system of claim 1 wherein said phenotypic profile analysis is automated immunofluorescent cell analysis means.
4. The system of claim 1 wherein said genotypic profile analysis means is FISH wherein probes used in said FISH are repeat-depleted.