Cell culture medium
The invention relates to nutrient media, in particular cell culture media, which contain at least one substance selected from the group comprising citric acid, succinic acid, malic acid, α-keto-glutaric acid, fumaric acid, oxalacetic acid, isocitric acid, oxalosuccinic acid, tartaric acid, lactic acid, adipic acid and mixtures thereof and salts, derivatives or complexes of these acids. The invention further relates to the use and methods of production of said cell culture media, methods of cultivation of a cell culture in a cell culture medium according to the invention and cells that can be obtained by said methods.
1. A cell line nutrient medium for the cultivation of a cell line, wherein the cell line nutrient medium contains at least one substance selected from the group consisting of succinic acid and salts and complexes of succinic acid, wherein the cell line nutrient medium contains glutamine at a concentration of less than 8 mmol/l, wherein the nutrient medium contains the at least one substance at a concentration of at least 0.2 g/l and at most 100 g/l and wherein the cell line nutrient medium is protein-free.
2. The nutrient medium according to claim 1 , wherein the nutrient medium contains the at least one substance at a concentration of at least 0.3 g/l and at most 50g/l.
3. The nutrient medium according to claim 1 , wherein the nutrient medium is glucose-free.
4. The nutrient medium according to claim 1 , wherein the nutrient medium is glutamine-free.
5. The nutrient medium according to claim 1 , wherein the nutrient medium is a liquid nutrient medium.
6. The nutrient medium according to claim 1 , wherein the nutrient medium is a basal medium with an osmolality of 240 to 360 mOsmol/kg H 2 O.
7. The nutrient medium according to claim 1 , wherein the nutrient medium is a feed medium with an osmolality of 150 to 1500 mOsmol/kg H 2 O.
8. The nutrient medium according to claim 1 , wherein the nutrient medium contains the at least one substance at a concentration of at least 1 g/l and at most 25 g/l.