Chimeric MSP-based malaria vaccine
View Patent ↗The invention provides an immunogenic composition comprising MSPk-8 linked to an antigen. Methods of using the composition to induce an immune response in an animal are also provided.
1. A composition comprising a chimeric protein, wherein said chimeric protein comprises Plasmodium merozoite surface protein-8 (MSP-8) linked to MSP-1 19 .
2. The composition of claim 1 , wherein said MSP-1 19 is covalently linked to MSP-8.
3. The composition of claim 1 , wherein MSP-8 and MSP-1 19 are P. falciparum or P . vivax sequences.
4. The composition of claim 2 , wherein said MSP-1 19 is linked to said MSP-8 via a linker selected from the group consisting of: a glycine linker, a glycine-rich linker and a glycine-serine linker.
5. A kit comprising the composition of claim 1 .
6. A vaccine comprising a composition comprising a chimeric protein, wherein said chimeric protein comprises Plasmodium merozoite surface protein-8 (MSP-8) linked to MSP-1 19 .
7. The vaccine of claim 6 , wherein said MSP-1 19 is covalently linked to said MSP-8.
8. The vaccine of claim 6 , wherein said MSP-8 and said MSP-1 19 are P. falciparum or P. vivax sequences.
9. The vaccine of claim 6 , further comprising a pharmaceutically acceptable carrier.
10. The vaccine of claim 6 , further comprising an adjuvant.
11. The vaccine of claim 7 , wherein MSP-1 19 is covalently linked to MSP-8 to form a fusion protein comprising the sequence of SEQ ID No. 23.