Prefilter system for biological systems
View Patent ↗The present invention is to a prefilter for affinity chromatography columns. The prefilter, positioned upstream of the column inlet, reduces the presence of non-specific binding (NSB) species that enter the system, thereby extending the yield, capacity and lifetime of the column. Suitable agents include but are not limited to hydrophobic entities; lipophilic entities; activated carbon; charged cation or anion entities; ligands; particles such as fumed silica, glass, controlled pore glass or derivitized version of each; silica or silicates; and combinations thereof. The material(s) can be incorporated into a variety of media such as fibers, beads, membranes and the like and then incorporated into a variety of device designs including lenticular pads, depth filters, bead containing columns, spiral wound devices, TFF devices and the like.
1. A tangential flow fluid (TFF) prefilter device for the selective upstream removal of non-specific binding constituents from a fluid stream containing protein during tangential flow fluid filtration, comprising
an inlet for receiving the protein containing fluid stream being purified;
a central distributor having two sides and in fluid communication with the inlet;
one or more layers of porous filter media located on each side of the distributer and in fluid communication with the distributer, each layer of porous filter media having a different pore size range and containing one or more nonspecific binding agents, wherein the nonspecific binding agents are particle-based materials selected from the group consisting of fumed silica, glass, silica, silicates, controlled pore glass, and combinations thereof,
a collection area in fluid communication with the porous filter media for receiving the protein containing fluid stream filtered through the media, and
an outlet in fluid communication with collection area, such that at least a portion of the protein containing fluid steam passes through the porous filter media on each tangential flow pass.
2. The prefilter device of claim 1 , wherein the porous filter media is selected from the group consisting of films, membranes, mats, monoliths, nonwovens, and woven fabrics.
3. The prefilter device of claim 1 , wherein the porous filter media is selected from the group consisting of cotton, polyolefins, polyethylene, polypropylene, polyvinyl alcohols, fluoropolymers, polyvinyl chlorides, polysulfones, polyarylsulfones, polyethersulfones, polyphenylsulphones, RIFE, polyesters, nylons, polyamides, polyimides, PVDF, celluloses, modified cellulosic materials, composite cellulosic materials, and cellulose acetate.
4. The prefilter device of claim 1 , wherein the wherein the particles is a bead having an average diameter of about 10 to about 1000 microns.