Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use
The present invention provides isolated polypeptides isolatable from a Staphylococcus spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.
1. An isolated polypeptide having immunogenic activity and a mass fingerprint when digested with trypsin that includes all polypeptide fragments present in a mass fingerprint of a reference polypeptide when digested with trypsin, wherein the reference polypeptide has a molecular weight of 38 kDa as determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and is expressed by Staphylococcus aureus ATCC strain 19636 when incubated in media comprising 2,2′-dipyridyl at a concentration of 1600 μM and not expressed at a detectable level when grown in the media without the 2,2′-dipyridyl.