Emulsion compositions
View Patent ↗An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.
1. A method of obtaining a gene product comprising:
(i) forming an emulsion comprising a surfactant, a hydrophobic phase and a hydrophilic phase, the hydrophilic phase comprising a plurality of microcapsules containing a functional in vitro eukaryotic expression system, wherein the surfactant is a chemically inert silicone-based surfactant;
(ii) allowing the gene product to be expressed by the expression system; and, optionally,
(iii) purifying the gene product.
2. The method of claim 1 , wherein the silicone-based surfactant is a silicone copolymer.
3. The method of claim 2 , wherein, the silicone-based surfactant is polysiloxane-polycetyl-polyethylene glycol copolymer.
4. The method of claim 1 , wherein the surfactant is provided at a v/v concentration in the oil phase of 4%.
5. The method of claim 1 , wherein the eukaryotic expression system comprises a mammalian cell lysate.
6. The method of claim 5 , wherein the lysate is a reticulocyte lysate.
7. The method of claim 5 , wherein the lysate is a rabbit reticulocyte lysate.
8. The method of claim 1 , wherein the emulsion is stable during incubation at 30° C. for at least one hour.
9. The method of claim 1 , wherein the hydrophobic phase comprises a mineral oil.