Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.
1 . An isolated polypeptide comprising an amino acid sequence selected from the group consisting of amino acids 21-263 of SEQ ID NO:18 and amino acids 21-263 of SEQ ID NO:27.
2 . The polypeptide of claim 1 , comprising the amino acid sequence of amino acids 21-263 of SEQ ID NO:18.
3 . The polypeptide of claim 1 , comprising the amino acid sequence of amino acids 21-263 of SEQ ID NO:27.
4 . The polypeptide of claim 1 , consisting of the amino acid sequence of amino acids 21-263 of SEQ ID NO:18.
5 . The polypeptide of claim 1 , consisting of the amino acid sequence of amino acids 21-263 of SEQ ID NO:27.
6 . A composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable excipient.
7 . A composition comprising the polypeptide of claim 2 and a pharmaceutically acceptable excipient.
8 . A composition comprising the polypeptide of claim 3 and a pharmaceutically acceptable excipient.
9 . A composition comprising the polypeptide of claim 4 and a pharmaceutically acceptable excipient.
10 . A composition comprising the polypeptide of claim 5 and a pharmaceutically acceptable excipient.
11 . A method of producing the polypeptide of claim 1 comprising:
(a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and
(b) isolating the polypeptide from the cell culture or cells in step (a).
12 . A method of producing the polypeptide of claim 2 comprising:
(a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and
(b) isolating the polypeptide from the cell culture or cells in step (a).
13 . A method of producing the polypeptide of claim 3 comprising:
(a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and
(b) isolating the polypeptide from the cell culture or cells in step (a).
14 . A method of producing the polypeptide of claim 4 comprising:
(a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and
(b) isolating the polypeptide from the cell culture or cells in step (a).
15 . A method of producing the polypeptide of claim 5 comprising:
(a) culturing a host cell comprising a polynucleotide sequence encoding said polypeptide, under conditions sufficient to express the polypeptide in said cell; and
(b) isolating the polypeptide from the cell culture or cells in step (a).
16 . A method of producing a composition comprising combining the polypeptide of claim 1 with a pharmaceutically acceptable excipient.
17 . A method of producing a composition comprising combining the polypeptide of claim 2 with a pharmaceutically acceptable excipient.
18 . A method of producing a composition comprising combining the polypeptide of claim 3 with a pharmaceutically acceptable excipient.
19 . A method of producing a composition comprising combining the polypeptide of claim 4 with a pharmaceutically acceptable excipient.
20 . A method of producing a composition comprising combining the polypeptide of claim 5 with a pharmaceutically acceptable excipient.