Composition comprising cross-species-specific antibodies and uses thereof
View Patent ↗The present invention relates to uses of bispecific antibodies exhibiting cross-species specificity for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of the same in non-human species and humans. The present invention moreover relates to methods for evaluating the in vivo safety and/or activity and/or pharmacokinetic profile of said bispecific anti-bodies exhibiting cross-species specificity. The present invention also relates to methods of measuring the biological activity and/or efficacy of such bispecific antibodies exhibiting cross-species specificity. In addition, the present invention relates to pharmaceutical compositions comprising bispecific single chain antibodies exhibiting cross-species specificity and to methods for the preparation of pharmaceutical compositions comprising said bispecific single chain antibodies exhibiting cross-species specificity for the treatment of diseases.
1. A pharmaceutical composition for the treatment of a human patient, comprising a bispecific single chain antibody which comprises
(i) a first binding domain binding to an epitope of human and non-chimpanzee primate CD3, and
(ii) a second binding domain binding to a cell surface antigen,
wherein the epitope is part of an amino acid sequence comprised in SEQ ID NOs. 134, 135 and 136, and comprises the amino acid sequence “FSEXE” (SEQ ID NO. 204), wherein “X” represents L (Leucine) or M (Methionine),
wherein the bispecific single chain antibody comprises an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NOs. 70, 72, 78 or 80;
(b) the amino acid sequence encoded by a nucleic acid sequence as shown in SEQ ID NOs. 69, 71, 77 or 79; and
(c) the amino acid sequence encoded by a nucleic acid sequence which is degenerate as a result of the genetic code to the nucleotide sequence of (b).
2. The pharmaceutical composition of claim 1 , wherein the non-chimpanzee primate is a baboon, marmoset or an old world monkey.
3. The pharmaceutical composition of claim 2 , wherein the old world monkey is a monkey of the macaque genus.
4. The pharmaceutical composition of claim 3 , wherein the monkey of the macaque genus is Assamese macaque ( Macaca assamensis ), Barbary macaque ( Macaca sylvanus ), Bonnet macaque ( Macaca radiata ), Booted or Sulawesi-Booted macaque ( Macaca ochreata ), Sulawesi-crested macaque ( Macaca nigra ), Formosan rock macaque ( Macaca cyclopsis ), Japanese snow macaque or Japanese macaque ( Macaca fuscata ), Cynomolgus monkey or crab-eating macaque or long-tailed macaque or Java macaque ( Macaca fascicularis ), Lion-tailed macaque ( Macaca silenus ), Pigtailed macaque ( Macaca nemestrina ), Rhesus macaque ( Macaca mulatta ), Tibetan macaque ( Macaca thibetana ), Tonkean macaque ( Macaca tonkeana ), Toque macaque ( Macaca sinica ), Stump-tailed macaque or Red-faced macaque or Bear monkey ( Macaca arctoides ), or Moor macaque ( Macaca maurus ).
5. The pharmaceutical composition of claim 1 , wherein at least one of said first or second binding domains is human, humanized, CDR-grafted and/or deimmunized, wherein the deimmunization is in the framework regions of the first and second binding domains.
6. A pharmaceutical composition according to claim 1 , further comprising a proteinaceous compound capable of providing an activation signal for immune effector cells.
7. A process for the production of a bispecific single chain antibody comprising culturing a host transformed or transfected with nucleic acid sequence encoding the bispecific single chain antibody of claim 1 or with a vector comprising the nucleic acid sequence, under conditions allowing the expression of the bispecific single chain antibody, and recovering the produced bispecific single chain antibody from the culture.
8. The pharmaceutical composition of claim 1 , which further comprises suitable formulations of carriers, stabilizers and/or excipients.
9. A kit comprising a bispecific single chain antibody which comprises
(i) a first binding domain binding to an epitope of human and non-chimpanzee primate CD3, and
(ii) a second binding domain binding to a cell surface antigen,
wherein the epitope is part of an amino acid sequence comprised in SEQ ID NOs. 134, 135 and 136, and comprises the amino acid sequence “FSEXE” (SEQ ID NO. 204), wherein “X” represents L (Leucine) or M (Methionine),
wherein the bispecific single chain antibody comprises an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NOs. 70, 72, 78 or 80;
(b) the amino acid sequence encoded by a nucleic acid sequence as shown in SEQ ID NOs. 69, 71, 77 or 79; and
(c) the amino acid sequence encoded by a nucleic acid sequence which is degenerate as a result of the genetic code to the nucleotide sequence of (b).