Means and methods for influencing the stability of cells
View Patent ↗The invention provides a method for influencing the stability of an antibody producing cell, comprising directly or indirectly influencing the amount of BCL6 and/or Blimp 1 expression product within said antibody producing cell. Stable antibody producing cells and cell lines are also provided, as well as methods for producing antibodies using such cells and/or cell lines.
1. A method for increasing the replicative life span of an antibody producing cell having a potential for expression of an amount of BCL6 and/or Blimp-1 expression, the method comprising:
providing the antibody producing cell with a nucleic acid molecule encoding a peptide comprising BCL6; and
increasing an expression level of Blimp-1 in the antibody producing cell by culturing the antibody producing cell in the present of IL-21 and/or IL-10, or by providing the antibody producing cell with STAT3.
2. The method according to claim 1 , wherein BCL6 and Blimp-1 are co-expressed in the antibody producing cell.
3. The method according to claim 1 , wherein the antibody producing cell produces antibodies against an antigen of interest.
4. The method according to claim 3 , wherein the antibody producing cell has been obtained from an individual, which individual had been previously exposed to the antigen of interest.
5. The method according to claim 1 , further comprising expressing a gene of the antibody producing cell encoding the Ig heavy chain and/or Ig light chain in a second cell.
6. A method for increasing the replicative life span of an antibody producing cell, which antibody producing cell co-expresses BCL6 and/or Blimp-1, the method comprising:
providing the antibody producing cell with a nucleic acid molecule encoding a peptide comprising BCL6; and
increasing an expression level of Blimp-1 in the antibody producing cell by culturing the antibody producing cell in the presence of IL-21 and/or IL-10, or by providing the antibody producing cell with STAT3;
wherein the antibody producing cell produces antibodies against an antigen of interest.
7. The method according to claim 6 , wherein the antibody producing cell is from a subject previously exposed to the antigen of interest.
8. A method for inclueasing an antibody producing cell's replicative life span, the method comprising:
providing a B cell with a polynucleotide encoding a peptide comprising the BCL6 to enhance the B cell's stability; and
culturing the B cell with IL-21, IL-10, and/or STAT3 to increase expression of Blimp-1 in the B cell,
so as to increase the B cell's replicative life span.
9. The method according to claim 1 , wherein the BCL6 forms a fusion protein.