Calibration device for a spectrophotometric system
View Patent ↗A method and apparatus for calibrating an NIRS system which includes a sensor portion and for evaluating an NIRS system for proper functioning is provided that includes an enclosure with at least two windows disposed in a wall of the enclosure. The windows allow the light source and one or more detectors of an NIRS system sensor to interface with the enclosure. One window is dedicated to the light source while each light detector has a window dedicated thereto. Thus, the enclosure includes a number of windows equal to the number of light detectors in the NIRS system sensor plus one. The inner surface of the wall(s) of the enclosure is of a light-absorbing color; e.g., black. A diffuse reflectance member of a light-reflecting color, e.g., white, is disposed in the enclosure spaced apart from the surface with the windows disposed therein.
1. A device for calibrating a spectrophotometric system having a sensor portion with a light source and at least one light detector, comprising:
an enclosure that includes:
at least two windows formed in the enclosure, the two windows having a predetermined spacing from one another;
each inside surface of the enclosure being of a color that absorbs a predetermined amount of light entering the enclosure through a first one of the windows and emanating from the light source; and
a diffuse reflectance member disposed inside the enclosure in a predetermined location therewithin to reflect a predetermined amount of the light entering the enclosure from the light source through the first one of the windows to the second one of the windows to the at least one light detector, an amount of the light reflected by the member to the second window being attenuated in magnitude as compared to an amount of light entering the enclosure through the first window, the diffuse reflectance member being of a color that reflects light.
2. The calibration device of claim 1 , where each of the at least two windows is covered by a light-diffusive layer.
3. The calibration device of claim 1 , where the enclosure further includes a third window formed therein.
4. The calibration device of claim 1 , where the color of each inside surface of the enclosure is black.
5. The calibration device of claim 1 , where the color of the diffuse reflectance member is white.
6. The calibration device of claim 1 , where the enclosure comprises a box.
7. The calibration device of claim 6 , where the at least two windows are located in a surface of the box, and where the diffuse reflectance member is disposed on an inside surface of the box that is opposite the surface in which the at least two windows are located.
8. The calibration device of claim 1 , where the diffuse reflectance member has a surface that reflects light.
9. The calibration device of claim 8 , where the reflecting surface of the member comprises a roughened material.
10. The calibration device of claim 8 , where an amount of surface area of the reflecting surface of the member determines at least in part the amount of the light from the light source that is reflected by the member to the second one of the windows.
11. The calibration device of claim 1 , where an amount of surface area of each inside surface of the enclosure determines at least in part an amount of the light from the light source that is absorbed by each inside surface of the enclosure and that is reflected by the member to the second one of the windows.
12. The calibration device of claim 1 , where the dimensions of the enclosure determine at least in part the amount of light from the light source that is reflected by the member to the second one of the windows.
13. A method for calibrating a spectrophotometric system having a sensor with a light source and at least one light detector, comprising the steps of:
calibrating a first spectrophotometric system;
providing a device for calibrating a spectrophotometric system, the calibrating device having an enclosure with an inside surface of a color that absorbs light, at least two windows formed in the enclosure, and a diffuse reflectance member disposed inside the enclosure;
interfacing the first spectrophotometric system with the calibrating device;
sending light from the light source of the first spectrophotometric system sensor through the first window into the enclosure where it is absorbed in part by the inside surface and is reflected in part by the diffuse reflectance member to the second window and to the at least one light detector, an amount of light present at the at least one light detector being attenuated as compared to an amount of light entering the enclosure from the light source;
interfacing an uncalibrated spectrophotometric system with the calibrating device;
sending light from the light source of the uncalibrated system through the first window into the enclosure where it is absorbed in part by the inside surface and is reflected in part by the diffuse reflectance member to the second window to the at least one light detector; and
calibrating the uncalibrated system by adjusting at least one predetermined internal parameter of the uncalibrated system until an amount of light present at the at least one light detector equals the calculated attenuated amount of light from the step of sending light performed with the first spectrophotometric system sensor interfaced with the calibrating device.
14. The calibration method of claim 13 , where the step of calibrating a first spectrophotometric system utilizes empirical data from a subject under study.
15. The calibration method of claim 13 , where the step of interfacing the first spectrophotometric system comprise the step of aligning the light source with a first one of the at least two windows so that light from the light source passes into the enclosure, and aligning the at least one light detector with a second one of the at least two windows so that light from inside the enclosure passes to the at least one light detector.
16. The calibration method of claim 13 , further comprising the step of locating the diffuse reflectance member inside the enclosure so that a predetermined amount of the light from the light source is reflected by the member to the second one of the at least two windows.
17. A disposable device for calibrating and evaluating a spectrophotometric system having a disposable sensor portion with a light source and at least one light detector, the disposable device comprising:
an enclosure that includes:
at least two windows formed in the enclosure, the two windows having a predetermined spacing from one another;
each inside surface of the enclosure being of a color that absorbs a predetermined amount of light entering the enclosure through a first one of the windows and emanating from the light source; and
a diffuse reflectance member disposed inside the enclosure in a predetermined location therewithin to reflect a predetermined amount of the light entering the enclosure from the light source through the first one of the windows to the second one of the windows to the at least one light detector, an amount of the light reflected by the member to the second window being attenuated in magnitude as compared to an amount of light entering the enclosure through the first window, the diffuse reflectance member being of a color that reflects light; and
an adhesive liner disposed between the sensor portion of the spectrophotometric system and the disposable device.
18. A method for calibrating a spectrophotometric system and for evaluating the operation of the spectrophotometric system, the spectrophotometric system having a disposable sensor with a light source and at least one light detector, the method comprising the steps of:
calibrating a first spectrophotometric system;
providing a disposable device for calibrating a spectrophotometric system, the disposable calibrating device having an enclosure with an inside surface of a color that absorbs light, at least two windows formed in the enclosure, and a diffuse reflectance member disposed inside the enclosure;
interfacing the disposable sensor portion of a first spectrophotometric system with the disposable calibrating device;
sending light from the light source of the first spectrophotometric system sensor through the first window into the enclosure where it is absorbed in part by the inside surface and is reflected in part by the diffuse reflectance member to the second window and to the at least one light detector, an amount of light present at the at least one light detector being attenuated as compared to an amount of light entering the enclosure from the light source;
interfacing the disposable sensor portion of an uncalibrated spectrophotometric system with the disposable calibrating device;
sending light from the light source of the uncalibrated system through the first window into the enclosure where it is absorbed in part by the inside surface and is reflected in part by the diffuse reflectance member to the second window to the at least one light detector; and
calibrating the uncalibrated system by adjusting at least one predetermined internal parameter of the uncalibrated system until an amount of light present at the at least one light detector equals the calculated attenuated amount of light from the step of sending light performed with the first spectrophotometric system sensor interfaced with the calibrating device.
19. The method of claim 18 , further comprising the step of evaluating the operation of the spectrophotometric system by comparing the amount of light present at the at least one light detector with a predetermined value and determining whether the result of the step of comparing is within an acceptable range.