Methods of evaluating BAFF
View Patent ↗The present disclosure provides compositions and methods relating to the evaluation of BAFF in a biological sample from a subject.
1. An isolated anti-BAFF antibody selected from the group consisting of:
(a) an antibody that binds BAFF independent of its physiological state;
(b) an antibody that binds to an epitope of BAFF outside of the binding sites of BCMA, TACI, and BR3;
(c) an antibody that competitively inhibits antibody B4H7 from binding to BAFF;
(d) an antibody that is competitively inhibited from binding to BAFF by antibody B4H7;
(e) an antibody that binds to the same site as antibody B4H7; and
(f) an antibody that comprises at least one complementarity determining region (CDR) that is identical to a corresponding CDR in antibody B4H7.
2. A method of evaluating total BAFF in a biological sample, the method comprising:
(a) contacting a biological sample with an anti-BAFF antibody according to claim 1 ; and
(b) evaluating the amount of BAFF bound by the antibody, wherein the amount of BAFF determined in (b) indicates total BAFF in the biological sample.
3. The method of claim 2 , wherein the biological sample or samples is or are obtained from a mammal that has an autoimmune disease.
4. The method of claim 2 , wherein the binding is evaluated qualitatively.
5. The method of claim 2 , wherein the binding is evaluated quantitatively.
6. The method of claim 2 , wherein the biological sample or samples comprises or comprise serum and the method comprises ELISA.
7. The method of claim 2 , wherein the biological sample or samples comprises or comprise blood and the method comprises flow cytometry.
8. The method of claim 2 , wherein the biological sample or samples comprises or comprise tissue and the method comprises immunohistochemistry.
9. The method of claim 2 , wherein the biological sample or samples comprises or comprise serum, blood, plasma, biopsy sample, tissue sample, cell suspension, biological fluid, saliva, oral fluid, cerebrospinal fluid, amniotic fluid, milk, colostrum, mammary gland secretion, lymph, urine, sweat, lacrimal fluid, gastric fluid, synovial fluid, or mucus.
10. The antibody of claim 1 , wherein the affinity of the antibody for free BAFF and the affinity of the antibody for BAFF bound to BAFF receptor differ by less than 30%.
11. The antibody of claim 1 , wherein the antibody binds to BAFF bound to BAFF receptor with an affinity of at least 10 6 M −1 .
12. A method of selecting the antibody of claim 1 , comprising determining whether the antibody binds BAFF independent of whether BAFF is bound to a receptor.
13. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody binds BAFF independent of its physiological state.
14. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody binds to an epitope of BAFF outside of the binding sites of BCMA, TACI, and BR3.
15. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody competitively inhibits antibody B4H7 from binding to BAFF.
16. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody is competitively inhibited from binding to BAFF by antibody B4H7.
17. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody binds to the same site as antibody B4H7.
18. An isolated anti-BAFF antibody according to claim 1 , wherein the antibody comprises at least one complementarity determining region (CDR) that is identical to a corresponding CDR in antibody B4H7.
19. An isolated B4H7 antibody as expressed by the hybridoma deposited under ATCC Deposit Designation Number PTA-7602.