Method for separating proteins from liquid media
View Patent ↗The invention relates to a method for separating proteins from liquid media, comprising providing a liquid medium containing proteins, providing a clay material which has: a specific surface greater than 150 m 2 /g, a pore volume greater than 0.35 ml/g, an ion exchange capacity greater than 40 meq/100 g, and a sediment volume in water of less than 15 ml/2 g, equilibrating said clay material to a pH of from 3.5 to 9.0, treating said liquid medium with the equilibrated clay material, and separating the purified, protein-depleted liquid medium from the clay material.
1. A method for separating proteins from liquid media, comprising
providing a liquid medium containing proteins,
providing a clay material which has:
a specific surface greater than 150 m 2 /g,
a pore volume greater than 0.35 ml/g,
an ion exchange capacity greater than 40 meq/100 g, and
a sediment volume in water of less than 15 ml/2 g,
equilibrating said clay material to a pH of from 3.5 to 9.0,
treating said liquid medium with the equilibrated clay material, and
separating the purified, protein-depleted liquid medium from the clay material.
2. The method as claimed in claim 1 , wherein the clay material has an Al 2 O 3 content of less than 11%, based on the anhydrous clay material (atro).
3. The method as claimed in claim 1 , wherein the clay material has an SiO 2 content of greater than 65% by weight, based on the anhydrous clay material (atro).
4. The method as claimed in claim 1 , wherein at least 40% of the pore volume of the clay material are provided by pores having a pore diameter of at least 14 nm.
5. The method as claimed in claim 1 , wherein the sediment volume of the clay material is less than 15 ml/2g, after said clay material has been left in water at room temperature for 3 days.
6. The method as claimed in claim 1 , wherein the clay material has not been subjected to any surface activation with acid.
7. The method as claimed in claim 1 , wherein the clay material is equilibrated with a buffer.
8. The method as claimed in claim 7 , wherein the buffer concentration is within a range from 30 to 100 mmol.
9. The method as claimed in claim 1 , wherein the clay material has been activated by treatment with an alkali metal-containing compound.
10. The method as claimed in claim 1 , wherein the proteins are removed by passing the liquid medium through a filter packing comprising the clay material.
11. The method as claimed in claim 1 , wherein the proteins are removed by passing the liquid medium through a chromatography column whose packing comprises the clay material.
12. The method as claimed in claim 1 , wherein the proteins bound to the clay material are eluted with an eluent, after the purified, protein-depleted liquid medium has been removed.
13. The method as claimed in claim 12 , wherein the eluent and the liquid medium have a different pH.
14. The method as claimed in claim 1 , wherein the clay material has a particle size of >45 μm.
15. The method as claimed in claim 1 , wherein the clay material mixture is used together with a further adsorptive material.
16. The method as claimed in claim 15 , wherein the further adsorptive material is selected from the group consisting of silica gel, cellulose and polyvinylpyrrolidone.
17. The method as claimed in claim 15 , wherein the clay material and the further adsorptive material are in a ratio of between 1:10 and 10:1, based on weight.
18. The method as claimed in claim 2 , wherein the clay material has an SiO 2 content of greater than 65% by weight, based on the anhydrous clay material (atro).
19. The method as claimed in claim 1 , wherein the sediment volume of the clay material is less than 10 ml/2g, after said clay material has been left in water at room temperature for 3 days.
20. The method as claimed in claim 1 , wherein the clay material has been activated by treatment with a sodium containing compound.