Wood and cell wall gene microarray
View Patent ↗The invention provides polynucleotide and polypeptide sequences isolated from P. radiate and E. grandis that are involved in wood and cell wall biosynthesis. Methods for using the sequences, along with constructs and transgenic plants, are provided also.
1. An isolated polynucleotide comprising a nucleic acid sequence at least 95% identical to SEQ ID NO: 93.
2. The isolated polynucleotide of claim 1 , wherein the polynucleotide is greater than or equal to 99%, 98%, 97%, 96%, or 95%, identical to SEQ ID NO: 93.
3. A DNA construct comprising the polynucleotide of claim 1 .
4. The DNA construct of claim 3 , further comprising a promoter, wherein the promoter and the polynucleotide are operably linked.
5. The DNA construct of claim 3 , wherein the polynucleotide encodes an RNA transcript.
6. The DNA construct of claim 5 , wherein the polynucleotide is in a sense or antisense orientation relative to the promoter.
7. The DNA construct of claim 5 , wherein the RNA transcript induces RNA interference of a polynucleotide having the nucleic acid sequence of SEQ ID NO: 93.
8. A plant cell transformed with the DNA construct of claim 3 .
9. A transgenic plant comprising the plant cell of claim 8 .
10. The transgenic plant of claim 9 , wherein a phenotype of the plant is different from a phenotype of a plant of the same species that has not been transformed with the DNA construct.
11. The transgenic plant of claim 9 , wherein the plant is a woody plant.
12. The transgenic plant of claim 11 , wherein the plant is a tree.
13. Wood obtained from a transgenic tree which has been transformed with the DNA construct of claim 3 .
14. Wood pulp obtained from a transgenic tree, wherein the wood pulp comprises the DNA construct of claim 3 .
15. The DNA construct of claim 5 , wherein the RNA transcript is a small interfering RNA.
16. A DNA construct, comprising a polynucleotide that encodes an RNA transcript, wherein the RNA transcript induces RNA interference of a polynucleotide having the nucleic acid sequence of SEQ ID NO: 93.