System and method for liquid delivery evaluation using solutions with multiple light absorbance spectral features
View Patent ↗A system and related method for improved liquid delivery evaluation using a solution containing one or more dyes such that the solution exhibits multiple distinct detectable light absorbance spectral features for calibrating or testing over extended volume or dilution ranges are described. The system includes: a photometric instrument capable of measuring optical absorbance at multiple wavelengths; one or more sample solutions to be dispensed using the liquid delivery system whose performance is being tested or calibrated; and vessels optionally pre-filled, or filled by the user, with diluent solution. The sample solutions contain one or more dyes, chosen so that multiple distinct detectable light absorbance spectral features, such as peaks and/or valleys and/or plateaus of the solution can be distinguished for volume or dilution ranges of interest. The concentrations of the dyes may be chosen so that a large volume delivery device is calibrated using a spectral feature in the solution with a low absorbance per unit pathlength, while a small volume delivery device is calibrated with the same sample solution but using a different spectral feature with a high absorbance per unit pathlength.
1. A method of determining a liquid volume of a sample, the method comprising the steps of:
a. providing in the sample a single solution including one or more dyes selected to produce in the sample multiple distinct detectable light absorbance spectral features, wherein the one or more dyes establish in the solution a first distinct detectable light absorbance spectral feature for a low volume or dilution range and a second distinct detectable light absorbance spectral feature for a high volume or dilution range;
b. measuring the light absorbance of multiple spectral features in the solution; and
c. determining the volume of the sample from the measured light absorbance of the sample.
2. The method of claim 1 wherein the multiple distinct detectable light absorbance spectral features are selected for measurement where there is a valley or plateau resulting in a slope close to zero.
3. The method of claim 1 comprising the step of selecting for the solution a first dye and a second dye, wherein the first dye establishes in the solution the first distinct detectable light absorbance spectral feature for the low volume or dilution range and the second dye establishes the second distinct detectable light absorbance spectral feature for the high volume or dilution range.
4. The method of claim 3 wherein the first dye is at a concentration giving a high absorbance per unit pathlength in the solution and the second dye is at a concentration giving a low absorbance per unit pathlength in the solution.
5. The method of claim 1 comprising the step of selecting for the solution a first dye and a second dye, wherein the first dye establishes in the solution the first distinct detectable light absorbance spectral feature for the first volume or dilution range and the second distinct detectable light absorbance spectral feature for the second volume or dilution range, and the second dye establishes a third distinct detectable light absorbance spectral feature for a third volume or dilution range and a fourth distinct detectable light absorbance spectral feature for a fourth volume or dilution range.
6. The method of claim 1 comprising the step of selecting for the solution only one dye to establish in the solution the first distinct detectable light absorbance spectral feature for the low volume or dilution range and the second distinct detectable light absorbance spectral feature for the high volume or dilution range.
7. The method of claim 1 comprising the step of selecting for the solution a first dye, a second dye, a third dye and a fourth dye, wherein the first dye establishes in the solution the first distinct detectable light absorbance spectral feature for the low volume or dilution range, the second dye establishes in the solution the second distinct detectable light absorbance spectral feature for the high volume or dilution range, the third dye establishes in the solution a third distinct detectable light absorbance spectral feature for a third volume or dilution range, and the fourth dye establishes in the solution a fourth distinct detectable light absorbance spectral feature for a fourth volume or dilution range.