Δ-9 elongases and their use in making polyunsaturated fatty acids
View Patent ↗The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.
1. A transformed microbial host cell comprising an isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having Δ9 elongase activity, wherein the polypeptide has at least 90% amino acid identity based on the Clustal V method of alignment, when compared to the amino acid sequence as set forth in SEQ ID No. 13 or SEQ ID No. 14;
(b) a nucleotide sequence encoding a polypeptide having Δ9 elongase activity, wherein the nucleotide sequence has at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence as set forth in SEQ ID No. 11, SEQ ID No. 12, or SEQ ID No. 26; or
(c) a complement of the nucleotide sequence of (a) or (b), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
2. The microbial host cell of claim 1 wherein the isolated polynucleotide encodes the amino acid sequence as set forth in SEQ ID NO:13 or SEQ ID NO:14.
3. The microbial host cell of claim 1 selected from the group consisting of yeast, algae, bacteria, euglenoids, stramenopiles and fungi.
4. The microbial host cell of claim 3 wherein the cell is a fungus of the genus Mortierella.
5. The microbial host cell of claim 3 wherein the cell is a stramenopiles selected from the group consisting of: Thraustochytrium and Schizochytrium.
6. The microbial host cell of claim 3 wherein the yeast is an oleaginous yeast.
7. The microbial host cell of claim 6 wherein the oleaginous yeast is selected from the group consisting of: Yarrowia, Candida, Rhodotorula, Rhodosporidium, Cryptococcus, Trichosporon and Lipomyces.
8. The microbial host cell of claim 1 , wherein the isolated nucleic acid molecule encoding a polypeptide having Δ9 elongase activity is set forth in SEQ ID NO:26 and wherein at least 98 codons of the encoded polypeptide are codon-optimized for expression in Yarrowia sp.