METHODS FOR IDENTIFYING CONDITIONS AFFECTING A CELL STATE
The present invention is directed to methods for identifying agents which affect cell state. The instant invention provides rapid and efficient methods for identifying agents which affect cell state. Methods are directed toward the screening of complex combinations of agents for their ability to affect cell state. In one embodiment, cells are incubated under suitable conditions and subjected to different agents. After an appropriate amount of time, the cells are assayed to determine what, if any, characteristics they possess. Cell characteristics can be organized in a manner such that different and novel cell states can be identified.
1 - 13 . (canceled)
14 . A method of identifying a cell state comprising:
providing an array of receptacles each containing cells to be investigated;
subjecting cells in different receptacles to different treatment conditions wherein said different treatment conditions are created with different agents and wherein said agents are selected from small molecules and proteins;
waiting a pre-determined period of time;
detecting the expression of at least 5 markers on said cells wherein said markers are receptors or ligands and wherein said detection is done with the aid of an antibody and a fluorescent marker;
creating a composite signature wherein said signature is a plot of marker expression versus marker;
calculating the Euclidean distance between the composite signatures of said different treatment conditions; and
ordering the measures of said Euclidean distance of all the composite signatures in a tartan plot based on similarity wherein a cluster in said tartan plot is indicative of a possible cell state.