Split spin centrifugation of test elements
View Patent ↗High-throughput centrifugation of batches of samples is achieved by dividing a single centrifugation run of a batch of samples into two or more staggered, discrete spins with fewer samples and in which the availability of centrifuge slots for loading or unloading of samples and the subsequent delivery of experimental results can be enhanced. The methodology is particularly useful in situations where multiple samples need to be processed rapidly, for example, as part of a STAT blood typing program in urgent care facilities prior to blood transfusion.
1. A method of batch centrifugation of test samples, said method comprising the steps of:
a) creating a plurality of smaller secondary batches from a primary batch of test samples, wherein said primary batch typically requires centrifugation for a time period equal to t in a primary centrifuge and the number of secondary batches is equal to y, which is at least 2;
b) loading the test samples of said secondary batches into x number of separate secondary centrifuges, in which x is at least 2;
c) staggering the operation of said x number of secondary centrifuges by a time period t/xy in relation to one another over a total centrifugation cycle for each secondary centrifuge that is equal to time period t;
d) pausing the operation of each of said staggered secondary centrifuges;
e) at least one of loading new test samples and unloading completed test samples from each of said paused secondary centrifuges during said pausing step; and
f) resuming said centrifugation of said paused secondary centrifuges, wherein the frequency of said unloading and reloading of each of said secondary centrifuges with said secondary batches is increased xy fold as compared to the frequency of unloading and reloading of a primary centrifuge loaded with a primary batch of test samples.
2. The method of claim 1 , wherein said test samples are disposed within sample receptacles.
3. The method of claim 2 , wherein said sample receptacles are bead cassettes.
4. The method of claim 2 , wherein said bead cassettes are gel cards.
5. The method of claim 2 , wherein said sample receptacles contain human samples.
6. The method of claim 2 , wherein said sample receptacles contain human blood samples.
7. The method of claim 2 , wherein said sample receptacles contain only emergency samples.
8. The method of claim 2 , wherein said sample receptacles comprise reagents for particle agglutination assays.
9. The method of claim 2 , wherein said sample receptacles comprise reagents for blood typing.
10. The method of claim 1 , wherein the number of secondary centrifuges is from 2 to 10.
11. The method of claim 1 , wherein the steps of centrifuging, loading and reloading are controlled by a control mechanism.
12. The method of claim 1 , wherein each of said secondary batches have the same number of test samples.
13. The method of claim 1 , wherein said test samples in each of said secondary batches are assessed for a result at the time of loading or reloading.
14. The method of claim 1 , wherein the centrifugation time of each of said secondary batches can be different from the centrifugation time of each of the other said secondary batches.
15. The method of claim 2 , wherein sample receptacles in each of said secondary batches are assessed for a result at said pausing step.