Kit for determining polysaccharide-binding activity of mononuclear cells present in peripheral blood
A kit for the determination of the ability of a peripheral blood mononuclear cell to bind to a polysaccharide herein provided comprises a detection reagent comprising a fluorescence-labeled immunostimulant polysaccharide; and a reference reagent comprising a fluorescence-labeled material of a polysaccharide different from that used in the fluorescence-labeled immunostimulant polysaccharide, and/or an immunostimulant polysaccharide which is not labeled with any fluorescent material and whose polysaccharide moiety is identical to that included in the fluorescence-labeled immunostimulant polysaccharide. The use of this kit would permit the estimation of the polysaccharide-binding ability of a peripheral blood mononuclear cell in a higher precision of estimation.
1. A kit for determining the activity of a peripheral blood mononuclear cell to bind to a polysaccharide, which comprises a detection reagent comprising a fluorescence-labeled immunostimulant polysaccharide; and a reference reagent,
wherein said reference reagent comprises a fluorescence-labeled material of a polysaccharide which is different from that in the fluorescence-labeled immunostimulant polysaccharide present in the detection reagent.
2. The kit of claim 1 , wherein the immunostimulant polysaceharide is a β-glucan.
3. The kit of claim 1 , wherein the immunostimulant polysaccharide is lentinan.
4. The kit of claim 1 , wherein the detection reagent and the reference reagent are liquid, respectively and contained in separate containers.
5. The kit of claim 4 , wherein said immunostimulant polysaccharide is a member selected from the group consisting of a β-glucan, an α-glucan, a hetero-glucan, and a protein-bound glucan.
6. The kit of claim 4 , wherein said immunostimulant polysaccharide is a β-glucan.
7. The kit of claim 4 , wherein said immunostimulant polysaccharide is a β-glucan selected from the group consisting of lentinan, zymosan, pachymaran, schizophyllan, pustulan, scleroglucan, and lichenan.
8. The kit of claim 4 , which further comprises a hemolysis/fixation liquid.
9. The kit of claim 8 , wherein said immunostimulant polysaccharide is a member selected from the group consisting of a β-glucan, an α-glucan, a hetero-glucan, and a protein-bound glucan.
10. The kit of claim 8 , wherein said immunostimulant polysaccharide is a β-glucan.
11. The kit of claim 8 , wherein said immunostimulant polysaccharide is a β-glucan selected from the group consisting of lentinan, zymosan, pachymaran, schizophyllan, pustulan, scieroglucan, and lichenan.
12. The kit of claim 8 , which further comprises a washing/analysis liquid.
13. The kit of claim 12 , wherein said immunostimulant polysaccharide is a member selected from the group consisting of a β-glucan, an α-glucan, a hetero-glucan, and a protein-bound glucan.
14. The kit of claim 12 wherein said immunostimulant polysaccharide is aβ-glucan.
15. The kit of claim 12 , wherein said immunostimulant polysaccharide is a β-glucan selected from the group consisting of lentinan, zymosan, pachymaran, schizophyllan, pustulan, scleroglucan, and lichenan.
16. The kit of claim 1 , wherein the reference reagent further comprises an immunostimulant polysaccharide which is not labeled with any fluorescent material and whose polysaccharide moiety is identical to that in the fluorescence-labeled immunostimulant polysaccharide in the detection reagent.
17. The kit of claim 1 , which further comprises a blood-collection tube.
18. The kit of claim 1 , wherein said immunostimulant polysaccharide is a member selected from the group consisting of a β-glucan, an α-glucan, a hetero-glucan, and a protein-bound glucan.
19. The kit of claim 1 , wherein said immunostimulant polysaccharide is a β-glucan selected from the group consisting of lentinan, zymosan, pachymaran, schizophyllan, pustulan, scieroglucan, and lichenan.