Oligonucleotides for amplifying
View Patent ↗Oligonucleotides useful for determining the presence of Trichomonas vaginalis in a test sample. The oligonucleotides may be incorporated into detection probes, helper probes, capture probes and amplification oligonucleotides, and used in various combinations thereof.
1. A set of oligonucleotides for use in amplifying a target region of nucleic acid derived from Trichomonas vaginalis , the set of oligonucleotides comprising a first amplification oligonucleotide, the base sequence of the first amplification oligonucleotide consisting of a target binding region at least 12 bases in length and, optionally, a 5′ sequence recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase, wherein the base sequence of the target binding region is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55 or SEQ ID NO:56.
2. The set of oligonucleotides of claim 1 , wherein the target binding region of the first amplification oligonucleotide is at least 18 bases in length.
3. The set of oligonucleotides of claim 1 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:54.
4. The set of oligonucleotides of claim 1 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55 or SEQ ID NO:56.
5. The set of oligonucleotides of claim 1 , wherein the base sequence of the target binding region is perfectly complementary to the base sequence of SEQ ID NO:54.
6. The set of oligonucleotides of claim 1 , wherein the first amplification oligonucleotide includes the 5′ sequence recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase.
7. The set of oligonucleotides of claim 1 further comprising a second amplification oligonucleotide, the base sequence of the second amplification oligonucleotide consisting of a target binding region at least 12 bases in length and, optionally, a 5′ sequence recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase, wherein the base sequence of the target binding region is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35 or SEQ ID NO:36.
8. The set of oligonucleotides of claim 7 , wherein the target binding region of the second amplification oligonucleotide is at least 18 bases in length.
9. The set of oligonucleotides of claim 7 , wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to a sequence contained with the base sequence of SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43 or SEQ ID NO:44.
10. The set of oligonucleotides of claim 9 , wherein the target binding region of the second amplification oligonucleotide is at least 18 bases in length.
11. The set of oligonucleotides of claim 9 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:54, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:44.
12. The set of oligonucleotides of claim 9 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55 or SEQ ID NO:56, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43 or SEQ ID NO:44.
13. The set of oligonucleotides of claim 12 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:54, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:44.
14. The set of oligonucleotides of claim 7 , wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to a sequence contained with the base sequence of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 or SEQ ID NO:48.
15. The set of oligonucleotides of claim 14 , wherein the target binding region of the second amplification oligonucleotide is at least 18 bases in length.
16. The set of oligonucleotides of claim 14 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:54, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:48.
17. The set of oligonucleotides of claim 16 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55 or SEQ ID NO:56, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47 or SEQ ID NO:48.
18. The set of oligonucleotides of claim 17 , wherein the base sequence of the target binding region of the first amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:54, and wherein the base sequence of the target binding region of the second amplification oligonucleotide is perfectly complementary to the base sequence of SEQ ID NO:48.
19. The set of oligonucleotides of claim 7 , wherein at least one of the first and second amplification oligonucleotides includes the 5′ sequence recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase.