Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye
View Patent ↗Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye such as a J-aggregated dye are described.
1. An article of manufacture comprising;
a support comprising a surface;
a chemiluminescent enhancing material on the surface of the support;
an energy acceptor dye on the surface of the support;
one or more biomolecular probes on the surface of the support; and
a molecular filter on the surface of the support;
wherein the molecular filter is selected from the group consisting of hemoglobin, dabcyl, DPX and DNP C2 Amine and wherein the energy acceptor dye is a J-aggregated dye.
2. The article of claim 1 , wherein the molecular filter is hemoglobin.
3. The article of claim 1 , wherein the chemiluminescent enhancing material is a cationic homopolymer or copolymer comprising positively charged onium groups.
4. The article of claim 3 , wherein the chemiluminescent enhancing material comprises poly(vinylbenzyldimethylbenzylammonium chloride) (BDMQ), poly(vinylbenzyltrimethylammonium chloride) (TMQ), poly(vinylbenzyltributylammonium chloride) (TBQ), poly(vinylbenzyltri(n-pentyl) ammonium chloride) (TPQ), poly(vinylbenzyltributylphosphonium chloride) (TB), poly(vinylbenzyltrioctylphosphonium chloride) (TO), two or more of any of the foregoing or a copolymer comprising one or more of the foregoing.
5. The article of claim 1 , wherein the support is a particle.
6. The article of claim 1 , wherein the support comprises latex, polystyrene, nylon, polyacrylamide or poly(styrenedivinyl benzene) beads.
7. The article of claim 1 , wherein the J-aggregated dye is a cyanine dye.
8. The article of claim 1 , wherein the one or more biomolecular probes comprise antibodies, polynucleotide, oligonucleotides, polypeptides, proteins, receptors, lectins or aptamers.
9. The article of claim 1 , wherein the support has an anionic surface, the chemiluminescent enhancing material is a cationic homopolymer or copolymer on the support surface and the J-aggregated dye is an anionic dye on the cationic homopolymer or copolymer.
10. The article of claim 9 , wherein the anionic dye is an anionic cyanine dye having the structure as set forth below: