IP Library › Granted Patent US 7,678,542
Granted Patent B2
US 7,678,542 · App. 12/132,198 · Granted Mar 16, 2010

Charge tags and the separation of nucleic acid molecules

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Quick Facts
Patent No.
US 7,678,542
App. No.
12/132,198
Granted
Mar 16, 2010
Kind
B2
Abstract

The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.

Claims (23)

1. A method of separating oligonucleotides, comprising the steps of:

a) treating a charge-balanced oligonucleotide containing a charge tag under conditions such that a charge-unbalanced oligonucleotide containing said charge tag is produced, wherein said charge-unbalanced oligonucleotide is contained in a reaction mixture; and

b) separating said charge-unbalanced oligonucleotide from said reaction mixture.

2. The method of claim 1 , wherein said conditions comprise treating said charge-balanced oligonucleotide with a reactant, wherein said reactant is selected from the group consisting of an enzyme, a chemical and a device.

3. The method of claim 1 , wherein said charge tag is attached to a terminal end of said oligonucleotide, said charge tag attached to a terminal end comprising a phosphate group and a positively charged moiety.

4. The method of claim 1 , wherein said charge tag comprises a dye.

5. The method of claim 4 , wherein said dye is positioned between said oligonucleotide and said positively charged moiety.

6. The method of claim 4 , wherein said positively charged moiety is positioned between said oligonucleotide and said dye.

7. The method of claim 3 , wherein said charge tag further comprises a second positively charged moiety.

8. The method of claim 1 , wherein said charge tag comprises one or more nucleotides.

9. The method of claim 8 , wherein said oligonucleotide comprises a sequence complementary to a target nucleic acid, wherein said one or more nucleotides of said charge tag are not complementary to said target nucleic acid.

10. The method of claim 3 , wherein said oligonucleotide comprises a first portion complementary to a target nucleic acid and a second portion that is not complementary to said target nucleic acid, wherein said second portion comprises said terminal end.

11. The method of claim 1 , wherein said charge-balanced oligonucleotide has a net neutral charge and wherein said charge-unbalanced oligonucleotide has a net positive charge.

12. The method of claim 1 , wherein said charge-balanced oligonucleotide has a net negative charge and wherein said charge-unbalanced oligonucleotide has a net positive charge.

13. The method of claim 1 , wherein said charge tag contains a primary amine.

14. The method of claim 1 , wherein said charge tag contains a secondary amine.

15. The method of claim 1 , wherein said charge tag contains a tertiary amine.

16. The method of claim 1 , wherein said charge tag contains an ammonium group.

17. The method of claim 1 , wherein said charge tag comprises a a positively charged moiety added by coupling of a positively charged phosphoramidite.

18. The method of claim 1 , wherein said charge tag comprises a a neutral moiety added by coupling of a neutral phosphoramidite.

19. The method of claim 1 , wherein said separating comprises capillary electrophoretic separation.

20. The method of claim 1 , wherein said separating comprises capillary zone electrophoretic separation.

21. The method of claim 1 , wherein said separating occurs in a microchannel.

Assignments (10)
RELEASE OF SECURITY INTEREST Recorded Apr 28, 2026
From: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
To: HOLOGIC, INC., ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO DIRECT RADIOGRAPHY CORP.; CYTYC CORPORATION, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO BIOLUCENT, LLC; CYTYC SURGICAL PRODUCTS, LLC, AS SUCCESSOR-BY-CONVERSION TO CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; GEN-PROBE INCORPORATED, ON ITS OWN BEHALF AND AS SUCCESSOR-BY-MERGER TO THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.
Reel/Frame 075566/0039 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 035820 FRAME: 0239. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY INTEREST RELEASE. Recorded Nov 9, 2017
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 044727/0529 →
CORRECTIVE ASSIGNMENT TO CORRECT THE INCORRECT PATENT NO. 8081301 PREVIOUSLY RECORDED AT REEL: 028810 FRAME: 0745. ASSIGNOR(S) HEREBY CONFIRMS THE SECURITY AGREEMENT. Recorded Nov 9, 2017
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 044432/0565 →
MERGER Recorded Oct 23, 2015
From: THIRD WAVE AGBIO, INC.; THIRD WAVE TECHNOLOGIES, INC.
To: GEN-PROBE INCORPORATED
Reel/Frame 036934/0796 →
SECURITY AGREEMENT Recorded Aug 7, 2015
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; DIRECT RADIOGRAPHY CORP.; GEN-PROBE INCORPORATED; GEN-PROBE PRODESSE, INC.; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.
To: BANK OF AMERICA, N.A., AS COLLATERAL AGENT
Reel/Frame 036307/0199 →
SECURITY INTEREST RELEASE REEL/FRAME 028810/0745 Recorded Jun 4, 2015
From: GOLDMAN SACHS BANK USA, AS COLLATERAL AGENT
To: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
Reel/Frame 035820/0239 →
SECURITY AGREEMENT Recorded Aug 1, 2012
From: HOLOGIC, INC.; BIOLUCENT, LLC; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS, LIMITED PARTNERSHIP; SUROS SURGICAL SYSTEMS, INC.; THIRD WAVE TECHNOLOGIES, INC.; GEN-PROBE INCORPORATED
To: GOLDMAN SACHS BANK USA
Reel/Frame 028810/0745 →
TERMINATION OF PATENT SECURITY AGREEMENTS AND RELEASE OF SECURITY INTERESTS Recorded Aug 26, 2010
From: GOLDMAN SACHS CREDIT PARTNERS, L.P., AS COLLATERAL AGENT
To: HOLOGIC, INC.; R2 TECHNOLOGY, INC.; SUROS SURGICAL SYSTEMS, INC.; BIOLUCENT, LLC; DIRECT RADIOGRAPHY CORP.; CYTYC SURGICAL PRODUCTS II LIMITED PARTNERSHIP; CYTYC SURGICAL PRODUCTS LIMITED PARTNERSHIP; CYTYC CORPORATION; CYTYC SURGICAL PRODUCTS III, INC.; CYTYC PRENATAL PRODUCTS CORP.; THIRD WAVE TECHNOLOGIES, INC.
Reel/Frame 024892/0001 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 2, 2008
From: LYAMICHEV, VICTOR; ALLAWI, HATIM T.; TAKOVA, TSETSKA; PRUDENT, JAMES R.; SKRZYPCZYNSKI, ZBIGNIEV; WAYLAND, SARAH R.; NERI, BRUCE P.; HALL, JEFF G.
To: THIRD WAVE TECHNOLOGIES, INC.
Reel/Frame 021620/0555 →
SECURITY AGREEMENT Recorded Jul 29, 2008
From: THIRD WAVE TECHNOLOGIES, INC.
To: GOLDMAN SACHS CREDIT PARTNERS L.P., AS COLLATERAL AGENT
Reel/Frame 021301/0780 →