Collagenolytic active enzyme containing compositions, and their use in the dental field
View Patent ↗The invention relates to a composition comprising at least one collagenolytic active enzyme with enzymatic activity at acidic pH for drilless enzymatic caries removal. Furthermore, the present invention relates to a process of producing this collagenolytic active enzyme comprising composition and to processes of removing caries. The invention also relates to the use of collagenolytic active enzyme comprising compositions for producing a treatment agent for dental applications.
1. A method of caries removal comprising the step of applying a composition to a tooth, said composition comprising:
one or more collagenolytic active enzymes;
at least one solvent;
at least one additive; and
at least one acid,
wherein at least one collagenolytic active enzyme is a serine-carboxyl proteinase, and wherein said composition has a collagenolytic activity of more than 5 units per milliliter and a pH value of about 1 to about 5.
2. The method of caries removal according to claim 1 further comprising the step of penetrating tooth enamel by treating a portion of the tooth with a mechanical tool before applying the composition to the treated tooth portion.
3. The method according to claim 1 , further comprising the step of removing an existing filling before applying the composition to the tooth.
4. The method according to claim 1 , further comprising the step of treating the tooth area affected by caries with a rotating drill before applying the composition to the affected tooth area.
5. The method according to claim 1 , wherein the at least one collagenolytic active enzyme is selected from the group consisting of Kumamolysin and ScP-A.
6. The method according to claim 1 , wherein the at least one collagenolytic active enzyme is obtainable from Bacillus novosp . MN-32 or Alicyclobacillus sendaiensis strain NTAP-1.
7. The method according to claim 1 , wherein the composition further comprises at least one further proteinase.
8. The method according to claim 7 , wherein one of the further proteinases is pepsin.
9. The method according to claim 1 , wherein at least one collagenolytic active enzyme is activated by Ca-ions.
10. The method according to claim 1 , wherein the at least one additive is selected from the group consisting of thickening agents, rheological additives, polyhydric alcohols, inhibitors, and buffers.
11. The method according to claim 1 , further comprising the step of rinsing the region of the tooth to which the composition was applied.
12. A method of caries removal comprising the step of applying a composition to a tooth, said composition comprising:
at least one collagenolytic active enzyme selected from the group consisting of Kumamolysin and ScP-A;
at least one solvent;
at least one additive;
at least one acid; and
pepsin,
wherein said composition has a collagenolytic activity of more than 5 units per milliliter and a pH value of about 1 to about 5.
13. The method of caries removal according to claim 12 , further comprising the step of penetrating tooth enamel by treating a portion of the tooth with a mechanical tool before applying the composition to the treated tooth portion.
14. The method according to claim 12 , further comprising the step of removing an existing filling before applying the composition to the tooth.
15. The method according to claim 12 , further comprising the step of treating the tooth area affected by caries with a rotating drill before applying the composition to the affected tooth area.
16. The method according to claim 12 , wherein the at least one collagenolytic active enzyme is obtainable from Bacillus novosp . MN-32 or Alicyclobacillus sendaiensis strain NTAP-1.
17. The method according to claim 12 , wherein the at least one collagenolytic active enzyme is activated by Ca-ions.
18. The method according to claim 12 , wherein the at least one additive is selected from the group consisting of thickening agents, rheological additives, polyhydric alcohols, inhibitors, and buffers.
19. The method according to claim 12 , further comprising the step of rinsing the region of the tooth to which the composition was applied.