Method of expressing long-chain prenyl diphosphate synthase
The present disclosure describes a method of producing a long-chain prenyl diphosphate synthase (in particular decaprenyl diphosphate synthase and solanesyl diphosphate synthase) using a gene and a protein which are required for enabling or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase as well as a method of efficiently producing a coenzyme Q having a long-chain isoprenoid in its side chain (in particular coenzyme Q 9 or coenzyme Q 10 ) using a microorganism. The present disclosure also describes a DNA having a base sequence shown under SEQ ID NO:1, 3 or 5 and a DNA sequence derived from the above base sequence by deletion, addition, insertion and/or substitution of one to several bases thereof, and coding for a protein enabling (functioning) or enhancing the activity expression of a eukaryote-derived long-chain prenyl diphosphate synthase in a cell of a host organism.
1. A transformant that is transformed with
at least one selected from the group consisting of the Schizosaccharomyces pombe DPS gene, the human DPS1 gene and the mouse SDS1 gene; and
a DNA defined under (a) or (b):
(a) a DNA encoding a protein which has the amino acid sequence of SEQ ID NO: 2;
(b) a DNA encoding a protein which has the amino acid sequence derived from the amino acid sequence of SEQ ID NO: 2 by deletion, addition, insertion or substitution of one amino acid thereof,
wherein the DNA defined under (a) or (b) enables or enhances the activity of the Schizosaccharomyces pombe DPS gene, the human DPS1 gene and/or the mouse SDS1 gene in a cell of a host organism, and
wherein the Schizosaccharomyces pombe DPS gene is amplifiable using polymerase chain reaction (PCR) primers of SEQ ID NOs: 13 and 14, the human DPS1 gene is amplifiable with PCR primers of SEQ ID NOs: 15 and 16, and the mouse SDS1 gene is amplifiable with PCR primers of SEQ ID NOs: 17 and 18.
2. The transformant according to claim 1 , the transformant being E. coli DH5α(pSTVDLP1, pBSDPS) (FERM BP-7548).
3. The transformant according to claim 1 , the transformant being E. coli DH5α(pSTVDLP1, pUhDPS1) (FERM BP-8025).
4. The transformant according to claim 1 , the transformant being E. coli DH5α(pSTVDLP1, pBmSDS1).
5. A method of producing coenzyme Q, comprising:
cultivating the transformant according to claim 1 in a medium to cause formation and accumulation of the coenzyme Q in the culture, and
recovering the coenzyme Q from the culture,
wherein a host microorganism of the transformant is capable of producing coenzyme Q.