Method of treating bone tissue
View Patent ↗In one embodiment, the method comprises providing tissue, preparing the tissue, and treating the tissue to improve remodeling characteristics of the tissue. The tissue may be, for example, cortical bone. Treating the tissue to improve remodeling characteristics may comprise heating the tissue, treating the tissue with a chemical, or other. Heating the tissue may be done in the absence of oxygen and may comprise heating the tissue in a vacuum, heating the tissue in an inert atmosphere, heating the tissue in a reducing atmosphere, coating the tissue with a protective coating and heating the tissue, or other. Further embodiments comprise treating the tissue in supercritical fluids, for example, to dry or virally inactivate the tissue.
1. A method of treating tissue comprising the steps of:
(a) providing tissue comprising cortical bone;
(b) drying the tissue by a method comprising critical point drying under pressure; and
(c) sterilizing the tissue by heating the tissue in an inert atmosphere comprising
nitrogen in the absence of oxygen,
wherein step (b) occurs prior to step (c) and the tissue maintains at least approximately 30% of its original strength.
2. The method of claim 1 , wherein the tissue further comprises cancellous bone.
3. The method of claim 1 , wherein heating the tissue disrupts collagen structure of the tissue.
4. The method of claim 1 , further comprising treating the tissue with collagenase.
5. The method of claim 1 , further comprising mechanically treating the tissue.
6. The method of claim 1 , further comprising chemically treating the tissue.
7. The method of claim 6 , wherein chemically treating the tissue comprises treating the tissue with an enzyme.
8. The method of claim 6 , wherein chemically treating the tissue comprises treating the tissue with a base.
9. The method of claim 1 , further comprising treating the tissue with energy.
10. The method of claim 1 , wherein the tissue is bone and further comprising at least partially demineralizing the bone after heating the tissue.
11. The method of claim 1 , further comprising drying the tissue after heating the tissue.
12. The method of claim 1 , further comprising defatting the tissue before heating the tissue.
13. The method of claim 1 , wherein critical point drying comprises:
providing tissue;
dehydrating the tissue with a CO 2 miscible solvent;
placing the tissue in a chamber;
filling the chamber with CO 2 ;
raising a temperature and the pressure of the chamber to approximately 31.1° C. and approximately 1100 psi; raising the temperature above approximately 31.1° C. and approximately 1100 psi; and releasing the CO 2 .
14. The method of claim 1 , wherein heating the tissue comprises gentle heating of the tissue.
15. The method of claim 14 , wherein gentle heating of the tissue denatures proteins within the tissue.
16. The method of claim 1 , wherein heating the tissue comprises heating the tissue at approximately 100° C.
17. The method of claim 1 , further comprising adding a bioactive agent to the tissue.
18. The method of claim 1 , further comprising forming an implant from the tissue.
19. The method of claim 18 , wherein forming an implant comprises particulating the tissue, mixing the tissue with a polymer, and treating particulated tissue and polymer mixture with supercritical CO 2 .
20. The method of claim 19 , wherein treating the particulated tissue and polymer mixture with supercritical CO 2 is done at approximately 105° C. and approximately 7,000psi.