Bio cell cleaning centrifuge and bio cell cleaning rotor used in the same
View Patent ↗According to an aspect of the present invention, there is provided a bio cell cleaning centrifuge including: a motor; a rotor rotated by the motor; holders that are equipped on the rotor and that hold test tubes to be pivotable toward a rotational radial direction of the rotor; a cleaning liquid distributor mounted on the rotor to supply a cleaning liquid to the test tubes; a locking mechanism that locks the holders so that the test tubes are in a vertical state with respect to the rotational radial direction; and a controller that controls the motor and the locking mechanism, wherein the holders are configured to hold the test tubes so that central axes thereof are inclined from a rotational axis direction toward a rotational tangent direction of the rotor.
1. A bio cell cleaning centrifuge comprising:
a motor that has a drive shaft;
a rotor that is engaged with the drive shaft to be rotated by the motor;
a plurality of holders that are equipped on the rotor in a circular array to be rotated together with the rotor and that hold a plurality of test tubes so as to be pivotable toward a rotational radial direction of the rotor, central axes of the holders being inclined toward a rotational tangent direction of the rotor;
a cleaning liquid distributor that is mounted on the rotor to be rotated together with the rotor and that supplies a cleaning liquid to the plurality of test tubes; and
a controller configured to control the motor to rotate in a first direction in an injection process in which a cleaning liquid is injected into the plurality of test tubes and to rotate in a second direction that is opposite to the first direction in a supernatant discharge process in which the cleaning liquid is discharged by rotating the rotor while locking the plurality of holders in the vertical state by a locking mechanism.
2. The bio cell cleaning centrifuge according to claim 1 ,
wherein the controller is configured to control the rotor such that the rotor rotates at a second speed in a centrifugal process in which floating cells in the plurality of test tubes are deposited at bottoms by rotating the rotor, and rotates at a first speed in the injection process, wherein the second speed is higher than the first speed.
3. The bio cell cleaning centrifuge according to claim 2 , wherein the controller is configured to control the rotor such that the rotor rotates at a third speed in the supernatant discharge process, and wherein the second speed is higher than the third speed.
4. The bio cell cleaning centrifuge according to claim 1 , wherein, each holder is configured to be inclined so that an upper end of the test tube is in a forwarder position in the rotational tangent direction than a lower end thereof in the injection process, and the upper end of the test tube is in a backwarder position in the rotational tangent direction than the lower end thereof in the supernatant discharge process.
5. A method of controlling a bio cell cleaning centrifuge, said bio cell cleaning centrifuge comprising:
a motor that has a drive shaft;
a rotor that is engaged with the drive shaft to be rotated by the motor;
plurality of holders that are equipped on the rotor in a circular array to be rotated together with the rotor and that hold a plurality of test tubes so as to be pivotable toward a rotational radial direction of the rotor, central axes of the holders being inclined toward a rotational tangent direction of the rotor;
a cleaning liquid distributor that is mounted on the rotor to be rotated together with the rotor and that supplies a cleaning liquid to the plurality of test tubes; and
a controller configured to control the motor to rotate in a first direction in an injection process in which a cleaning liquid is injected into the plurality of test tubes and to rotate in a second direction that is opposite to the first direction in a supernatant discharge process in which the cleaning liquid is discharged by rotating the rotor while locking the plurality of holders in the vertical state by a locking mechanism:
the method comprises steps of:
injecting the cleaning liquid into the plurality of test tubes while rotating the rotor in a first rotational direction at a first speed and holding the test tubes in an inclined state such that an upper end of the test tube is in a forwarder position in a rotational tangent direction than a lower end thereof;
depositing floating cells in the plurality of test tubes at bottoms thereof while rotating the rotor at a second speed; and
discharging a supernatant of the cleaning liquid in the plurality of test tubes while rotating the rotor in a second rotational direction that is opposite to the first rotational direction at a third speed and holding the test tubes in an inclined state such that an upper end of the test tube is in a backwarder position in the rotational tangent direction than a lower end thereof.
6. The method according to claim 5 , wherein the second speed is higher than the first speed.
7. The method according to claim 5 , wherein the second speed is higher than the third speed.