Nanoparticles for protein drug delivery
View Patent ↗The invention discloses the nanoparticles composed of chitosan, poly-glutamic acid, and at least one bioactive agent characterized with a positive surface charge and their enhanced permeability for paracellular drug delivery.
1. A method of enhancing paracellular permeability of at least one bioactive agent through epithelial cells in a patient comprising administering into the patient bioactive nanoparticles with a dosage effective for enhancing said permeability, wherein said bioactive nanoparticles comprise a shell substrate of chitosan, said bioactive agent and a core substrate selected from the group consisting of γ-PGA, α-PGA, water soluble salts of PGA and metal salts of PGA.
2. The method of claim 1 , wherein said bioactive agent is conjugated with said core substrate.
3. The method of claim 1 , wherein said bioactive a gent is a hormone.
4. The method of claim 1 , wherein said bioactive agent is a growth hormone.
5. The method of claim 1 , wherein said bioactive agent is a tyrotropin releasing hormone.
6. The method of claim 1 , wherein a substantial portion of a surface of said nanoparticles is characterized with a positive surface charge.
7. The method of claim 1 , wherein said nanoparticles are in a spheroidal shape having a mean particle size of about 50 nanometers to 400 nanometers.
8. The method of claim 1 , wherein said core substrate is heparin.
9. The method of claim 1 , wherein said bioactive nanoparticles are treated with a coating.
10. The method of claim 1 , wherein said method comprises administering said nanoparticles into a blood vessel of the patient.
11. The method of claim 1 , wherein said bioactive agent comprises an antagonist for Alzheimer's disease.
12. The method of claim 1 , wherein said bioactive nanoparticles are encapsulated in capsules.
13. The method of claim 12 , wherein said capsule is treated with enteric coating.
14. The method of claim 1 , wherein said bioactive nanoparticles are encapsulated in hard-filled capsules.
15. The method of claim 1 , wherein said method of administering said bioactive nanoparticles is via an oral route.
16. The method of claim 1 , wherein said nanoparticles are formed via a simple and mild ionic-gelation method.
17. The method of claim 1 , wherein said bioactive agent is insulin.
18. The method of claim 1 , wherein said chitosan is trimethyl chitosan.
19. The method of claim 1 , wherein said nanoparticles further comprise at least one paracellular transport enhancer.